Transretinal degeneration in ageing human retina: A multiphoton microscopy analysis

Y. Lei, N. Garrahan, B. Hermann, Michael P Fautsch, D. H. Johnson, M. R. Hernandez, M. Boulton, J. E. Morgan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Aim: Retinal cell remodelling has been reported as a consistent feature of ageing. However, the degree to which this results in transretinal degeneration is unclear. To address this, the authors used multiphoton microscopy to quantify retinal degeneration in post-mortem human eyes of two age groups. Methods: Retinas from six young subjects (18-33 years old) and six older subjects (74-90 years old) were prepared as wholemount preparations. All retinas were stained with 4,6-diamidino-2-phenylindole and imaged by multiphoton confocal microscopy to quantify neuron densities in the retinal ganglion cell layer (RGCL), inner nuclear layer (INL) and outer nuclear layer (ONL). Neurons were counted using automated cell identification algorithms. All retinas were imaged hydrated to minimise tissue artefacts. Results: In both groups, 56% of the area within the central 4 mm eccentricity and 27% of the area with eccentricity between 4 mm and 7 mm were imaged. Compared with young subjects, the peak RGCL neuron loss in the aged subjects (25.5%) was at 1 mm eccentricity. INL and ONL neuron densities significantly decreased at 1-2 mm eccentricity (8.7%) and 0.5-4 mm eccentricity (15.6%) respectively (P <0.05). The reduction in neuron density in the INL corresponded, spatially, to the region with the greatest neuron loss in the RGCL and ONL. Conclusions: This is the first study to correlate neurodegeneration in different populations of cells in the ageing retinas. These data confirm that the greatest neuronal loss occurs in the RGCL and ONL in human ageing retinas, whereas the INL is relatively preserved.

Original languageEnglish (US)
Pages (from-to)727-730
Number of pages4
JournalBritish Journal of Ophthalmology
Volume95
Issue number5
DOIs
StatePublished - May 2011

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Retina
Microscopy
Retinal Ganglion Cells
Neurons
Retinal Degeneration
Cell Aging
Confocal Microscopy
Artifacts
Age Groups
Population

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Lei, Y., Garrahan, N., Hermann, B., Fautsch, M. P., Johnson, D. H., Hernandez, M. R., ... Morgan, J. E. (2011). Transretinal degeneration in ageing human retina: A multiphoton microscopy analysis. British Journal of Ophthalmology, 95(5), 727-730. https://doi.org/10.1136/bjo.2010.180869

Transretinal degeneration in ageing human retina : A multiphoton microscopy analysis. / Lei, Y.; Garrahan, N.; Hermann, B.; Fautsch, Michael P; Johnson, D. H.; Hernandez, M. R.; Boulton, M.; Morgan, J. E.

In: British Journal of Ophthalmology, Vol. 95, No. 5, 05.2011, p. 727-730.

Research output: Contribution to journalArticle

Lei, Y, Garrahan, N, Hermann, B, Fautsch, MP, Johnson, DH, Hernandez, MR, Boulton, M & Morgan, JE 2011, 'Transretinal degeneration in ageing human retina: A multiphoton microscopy analysis', British Journal of Ophthalmology, vol. 95, no. 5, pp. 727-730. https://doi.org/10.1136/bjo.2010.180869
Lei, Y. ; Garrahan, N. ; Hermann, B. ; Fautsch, Michael P ; Johnson, D. H. ; Hernandez, M. R. ; Boulton, M. ; Morgan, J. E. / Transretinal degeneration in ageing human retina : A multiphoton microscopy analysis. In: British Journal of Ophthalmology. 2011 ; Vol. 95, No. 5. pp. 727-730.
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