TY - JOUR
T1 - Translational opportunities of single-cell biology in atherosclerosis
AU - De Winther, Menno P.J.
AU - Bäck, Magnus
AU - Evans, Paul
AU - Gomez, Delphine
AU - Goncalves, Isabel
AU - Jørgensen, Helle F.
AU - Koenen, Rory R.
AU - Lutgens, Esther
AU - Norata, Giuseppe Danilo
AU - Osto, Elena
AU - Dib, Lea
AU - Simons, Michael
AU - Stellos, Konstantinos
AU - Ylä-Herttuala, Seppo
AU - Winkels, Holger
AU - Bochaton-Piallat, Marie Luce
AU - Monaco, Claudia
N1 - Publisher Copyright:
© 2022 The Author(s). Published by Oxford University Press on behalf of the European Society of Cardiology.
PY - 2023/4/7
Y1 - 2023/4/7
N2 - The advent of single-cell biology opens a new chapter for understanding human biological processes and for diagnosing, monitoring, and treating disease. This revolution now reaches the field of cardiovascular disease (CVD). New technologies to interrogate CVD samples at single-cell resolution are allowing the identification of novel cell communities that are important in shaping disease development and direct towards new therapeutic strategies. These approaches have begun to revolutionize atherosclerosis pathology and redraw our understanding of disease development. This review discusses the state-of-the-art of single-cell analysis of atherosclerotic plaques, with a particular focus on human lesions, and presents the current resolution of cellular subpopulations and their heterogeneity and plasticity in relation to clinically relevant features. Opportunities and pitfalls of current technologies as well as the clinical impact of single-cell technologies in CVD patient care are highlighted, advocating for multidisciplinary and international collaborative efforts to join the cellular dots of CVD.
AB - The advent of single-cell biology opens a new chapter for understanding human biological processes and for diagnosing, monitoring, and treating disease. This revolution now reaches the field of cardiovascular disease (CVD). New technologies to interrogate CVD samples at single-cell resolution are allowing the identification of novel cell communities that are important in shaping disease development and direct towards new therapeutic strategies. These approaches have begun to revolutionize atherosclerosis pathology and redraw our understanding of disease development. This review discusses the state-of-the-art of single-cell analysis of atherosclerotic plaques, with a particular focus on human lesions, and presents the current resolution of cellular subpopulations and their heterogeneity and plasticity in relation to clinically relevant features. Opportunities and pitfalls of current technologies as well as the clinical impact of single-cell technologies in CVD patient care are highlighted, advocating for multidisciplinary and international collaborative efforts to join the cellular dots of CVD.
KW - Atherosclerosis
KW - Endothelial cell
KW - Lymphocyte
KW - Macrophage
KW - Single-cell biology
KW - Smooth muscle cell
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U2 - 10.1093/eurheartj/ehac686
DO - 10.1093/eurheartj/ehac686
M3 - Review article
C2 - 36478058
AN - SCOPUS:85152164399
SN - 0195-668X
VL - 44
SP - 1216
EP - 1230
JO - European heart journal
JF - European heart journal
IS - 14
ER -