Transit of solids through the human colon: Regional quantification in the unprepared bowel

M. Proano, Michael Camilleri, S. F. Phillips, M. L. Brown, G. M. Thomforde

Research output: Contribution to journalArticle

190 Citations (Scopus)

Abstract

We used a noninvasive method to label the solid phase of contents in the unprepared human colon. 111In-labeled Amberlite pellets (0.5-1.8 mm diam) were placed in a gelatin capsule that was then coated with a pH-sensitive polymer (methacrylate). In vitro, the capsules disintegrated in simulated small bowel contents within 1-2 h; when ingested by healthy subjects, capsules released radiolabel in the distal ileum or proximal colon in 13 of 15 subjects. Transit of 111In-pellets through the unprepared colon could then be quantitated radioscintigraphically. Segmental transit was defined in the ascending (AC), transverse (TC), descending (DC), and rectosigmoid (RS) colon. Radioactivity was also quantitated in stools. At 12 h, radioactivity was most obvious in the AC (59 ± 11%, mean ± SE) and the TC (21 ± 6%); at 24 h, counts were distributed equally between AC, TC, and stools (P > 0.005); by 48 h, 56 ± 11% counts had been excreted, although 30 ± 10% remained in the TC. At 24 and 48 h, the amount in DC or RS was lower (P <0.05) than in the TC or in stools. Emptying of the AC was characterized by an initial lag period, when no counts emptied into the TC, followed by a period of emptying that was approximately linear. Thus this simple approach is able to label contents in the healthy human colon. The ascending and transverse colon appear to be sites of storage of solid residue, whereas the left colon and rectosigmoid function mainly as conduits.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume258
Issue number6 21-6
StatePublished - 1990

Fingerprint

Colon
Capsules
Radioactivity
Descending Colon
Ascending Colon
Transverse Colon
Methacrylates
Gelatin
Ileum
Healthy Volunteers
Polymers

Keywords

  • ascending colon emptying
  • colonic transit
  • particle size discrimination
  • solid residue

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Cite this

Transit of solids through the human colon : Regional quantification in the unprepared bowel. / Proano, M.; Camilleri, Michael; Phillips, S. F.; Brown, M. L.; Thomforde, G. M.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 258, No. 6 21-6, 1990.

Research output: Contribution to journalArticle

@article{d5869b057f1a4f9989121c823aaaf034,
title = "Transit of solids through the human colon: Regional quantification in the unprepared bowel",
abstract = "We used a noninvasive method to label the solid phase of contents in the unprepared human colon. 111In-labeled Amberlite pellets (0.5-1.8 mm diam) were placed in a gelatin capsule that was then coated with a pH-sensitive polymer (methacrylate). In vitro, the capsules disintegrated in simulated small bowel contents within 1-2 h; when ingested by healthy subjects, capsules released radiolabel in the distal ileum or proximal colon in 13 of 15 subjects. Transit of 111In-pellets through the unprepared colon could then be quantitated radioscintigraphically. Segmental transit was defined in the ascending (AC), transverse (TC), descending (DC), and rectosigmoid (RS) colon. Radioactivity was also quantitated in stools. At 12 h, radioactivity was most obvious in the AC (59 ± 11{\%}, mean ± SE) and the TC (21 ± 6{\%}); at 24 h, counts were distributed equally between AC, TC, and stools (P > 0.005); by 48 h, 56 ± 11{\%} counts had been excreted, although 30 ± 10{\%} remained in the TC. At 24 and 48 h, the amount in DC or RS was lower (P <0.05) than in the TC or in stools. Emptying of the AC was characterized by an initial lag period, when no counts emptied into the TC, followed by a period of emptying that was approximately linear. Thus this simple approach is able to label contents in the healthy human colon. The ascending and transverse colon appear to be sites of storage of solid residue, whereas the left colon and rectosigmoid function mainly as conduits.",
keywords = "ascending colon emptying, colonic transit, particle size discrimination, solid residue",
author = "M. Proano and Michael Camilleri and Phillips, {S. F.} and Brown, {M. L.} and Thomforde, {G. M.}",
year = "1990",
language = "English (US)",
volume = "258",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "6 21-6",

}

TY - JOUR

T1 - Transit of solids through the human colon

T2 - Regional quantification in the unprepared bowel

AU - Proano, M.

AU - Camilleri, Michael

AU - Phillips, S. F.

AU - Brown, M. L.

AU - Thomforde, G. M.

PY - 1990

Y1 - 1990

N2 - We used a noninvasive method to label the solid phase of contents in the unprepared human colon. 111In-labeled Amberlite pellets (0.5-1.8 mm diam) were placed in a gelatin capsule that was then coated with a pH-sensitive polymer (methacrylate). In vitro, the capsules disintegrated in simulated small bowel contents within 1-2 h; when ingested by healthy subjects, capsules released radiolabel in the distal ileum or proximal colon in 13 of 15 subjects. Transit of 111In-pellets through the unprepared colon could then be quantitated radioscintigraphically. Segmental transit was defined in the ascending (AC), transverse (TC), descending (DC), and rectosigmoid (RS) colon. Radioactivity was also quantitated in stools. At 12 h, radioactivity was most obvious in the AC (59 ± 11%, mean ± SE) and the TC (21 ± 6%); at 24 h, counts were distributed equally between AC, TC, and stools (P > 0.005); by 48 h, 56 ± 11% counts had been excreted, although 30 ± 10% remained in the TC. At 24 and 48 h, the amount in DC or RS was lower (P <0.05) than in the TC or in stools. Emptying of the AC was characterized by an initial lag period, when no counts emptied into the TC, followed by a period of emptying that was approximately linear. Thus this simple approach is able to label contents in the healthy human colon. The ascending and transverse colon appear to be sites of storage of solid residue, whereas the left colon and rectosigmoid function mainly as conduits.

AB - We used a noninvasive method to label the solid phase of contents in the unprepared human colon. 111In-labeled Amberlite pellets (0.5-1.8 mm diam) were placed in a gelatin capsule that was then coated with a pH-sensitive polymer (methacrylate). In vitro, the capsules disintegrated in simulated small bowel contents within 1-2 h; when ingested by healthy subjects, capsules released radiolabel in the distal ileum or proximal colon in 13 of 15 subjects. Transit of 111In-pellets through the unprepared colon could then be quantitated radioscintigraphically. Segmental transit was defined in the ascending (AC), transverse (TC), descending (DC), and rectosigmoid (RS) colon. Radioactivity was also quantitated in stools. At 12 h, radioactivity was most obvious in the AC (59 ± 11%, mean ± SE) and the TC (21 ± 6%); at 24 h, counts were distributed equally between AC, TC, and stools (P > 0.005); by 48 h, 56 ± 11% counts had been excreted, although 30 ± 10% remained in the TC. At 24 and 48 h, the amount in DC or RS was lower (P <0.05) than in the TC or in stools. Emptying of the AC was characterized by an initial lag period, when no counts emptied into the TC, followed by a period of emptying that was approximately linear. Thus this simple approach is able to label contents in the healthy human colon. The ascending and transverse colon appear to be sites of storage of solid residue, whereas the left colon and rectosigmoid function mainly as conduits.

KW - ascending colon emptying

KW - colonic transit

KW - particle size discrimination

KW - solid residue

UR - http://www.scopus.com/inward/record.url?scp=0025350430&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025350430&partnerID=8YFLogxK

M3 - Article

C2 - 2360632

AN - SCOPUS:0025350430

VL - 258

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 6 21-6

ER -