@inbook{75d8baacaf684a9bacb43de44c29ae87,
title = "Transgenic zebrafish using transposable elements",
abstract = "DNA transposons are effective chromosomal engineering vehicles for making transgenic zebrafish. We describe both autonomous and non-autonomous transposable elements, and we compare and contrast popular transposon systems. The Tol2 system is a robust gene transfer tool and has been selected as the primary transposon platform, facilitating the development of an array of reagents readily shared within the zebrafish community. We present common transposon and transposase vectors within the field based on the Tol2 system. We describe methods with a high success rate of generating transgenic zebrafish using Tol2 vectors, including key quality control steps during the transgenesis process. Together, these data should enable the ready generation of transgenic zebrafish for scientific inquiry.",
keywords = "Tol2, Transgenesis, Transgenic, Transposon, Zebrafish",
author = "Clark, {Karl J.} and Urban, {Mark D.} and Skuster, {Kimberly J.} and Ekker, {Stephen C.}",
note = "Funding Information: This work was supported by the Mayo Foundation and the NIH (grants DA14546, GM63904 and DK84567). ",
year = "2011",
doi = "10.1016/B978-0-12-374814-0.00008-2",
language = "English (US)",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc",
pages = "137--149",
booktitle = "Methods in Cell Biology",
}