Transgenic zebrafish using transposable elements

Karl J. Clark, Mark D. Urban, Kimberly J. Skuster, Stephen C. Ekker

Research output: Chapter in Book/Report/Conference proceedingChapter

43 Scopus citations

Abstract

DNA transposons are effective chromosomal engineering vehicles for making transgenic zebrafish. We describe both autonomous and non-autonomous transposable elements, and we compare and contrast popular transposon systems. The Tol2 system is a robust gene transfer tool and has been selected as the primary transposon platform, facilitating the development of an array of reagents readily shared within the zebrafish community. We present common transposon and transposase vectors within the field based on the Tol2 system. We describe methods with a high success rate of generating transgenic zebrafish using Tol2 vectors, including key quality control steps during the transgenesis process. Together, these data should enable the ready generation of transgenic zebrafish for scientific inquiry.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc
Pages137-149
Number of pages13
DOIs
StatePublished - 2011

Publication series

NameMethods in Cell Biology
Volume104
ISSN (Print)0091-679X

Keywords

  • Tol2
  • Transgenesis
  • Transgenic
  • Transposon
  • Zebrafish

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Clark, K. J., Urban, M. D., Skuster, K. J., & Ekker, S. C. (2011). Transgenic zebrafish using transposable elements. In Methods in Cell Biology (pp. 137-149). (Methods in Cell Biology; Vol. 104). Academic Press Inc. https://doi.org/10.1016/B978-0-12-374814-0.00008-2