TY - JOUR
T1 - Transgenic mice expressing a human poliovirus receptor
T2 - A new model for poliomyelitis
AU - Ren, Ruibao
AU - Costantini, Frank
AU - Gorgacz, Edward J.
AU - Lee, James J.
AU - Racaniello, Vincent R.
N1 - Funding Information:
We thank Chu-Hui Peng for technical assistance. This work was supported by grants to V. R. R. from the American Cancer Society and from the World Health Organization as part of its Programme for Vaccine Development. J. L. L. is a postdoctoral fellow of the American Cancer Society. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 16 USC Section 1734 solely to indicate this fact.
PY - 1990/10/19
Y1 - 1990/10/19
N2 - A human poliovirus receptor (PVR) gene was used to generate transgenic mice that express PVR transcripts and poliovirus binding sites in a wide range of tissues: Intracerebral inoculation of PVR transgenic mice with poliovirus type 1, Mahoney strain, resulted in viral replication in the brain and spinal cord and development of paralytic poliomyelitis. P1/Mahoney did not replicate or cause paralysis in nontransgenic mice. PVR transgenic mice failed to develop clinical disease when inoculated intracerebrally with the live attenuated Sabin type 1 vaccine strain. These results demonstrate that the PVR is the major determinant of poliovirus host range in mice. Transgenic mice expressing human PVR should be useful for studying poliovirus neurovirulence, attenuation, and tissue tropism, and for development and testing of poliovirus vaccine strains.
AB - A human poliovirus receptor (PVR) gene was used to generate transgenic mice that express PVR transcripts and poliovirus binding sites in a wide range of tissues: Intracerebral inoculation of PVR transgenic mice with poliovirus type 1, Mahoney strain, resulted in viral replication in the brain and spinal cord and development of paralytic poliomyelitis. P1/Mahoney did not replicate or cause paralysis in nontransgenic mice. PVR transgenic mice failed to develop clinical disease when inoculated intracerebrally with the live attenuated Sabin type 1 vaccine strain. These results demonstrate that the PVR is the major determinant of poliovirus host range in mice. Transgenic mice expressing human PVR should be useful for studying poliovirus neurovirulence, attenuation, and tissue tropism, and for development and testing of poliovirus vaccine strains.
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U2 - 10.1016/0092-8674(90)90168-E
DO - 10.1016/0092-8674(90)90168-E
M3 - Article
C2 - 2170026
AN - SCOPUS:0025183917
SN - 0092-8674
VL - 63
SP - 353
EP - 362
JO - Cell
JF - Cell
IS - 2
ER -