@inbook{67895c45b8744038a116e593b8f35092,
title = "Transgenesis in Mouse Embryonic Stem Cells",
abstract = "Traditionally, transgenic mice are generated by pronuclear injection of exogenous DNA. This technique has several limitations, including limited control over transgene expression, transgene cytotoxicity, promiscuity and silencing, and founder mouse sterility. Here we describe two protocols to generate transgenic mice from ES cell clones carrying stably integrated exogenous DNA with inducible transgene expression.",
keywords = "Cre recombinase, Doxycycline, ES cells, Inducible expression, Transgenic mice",
author = "{van Ree}, Janine and Wei Zhou and Ming Li and {van Deursen}, {Jan M.}",
note = "Publisher Copyright: {\textcopyright} 2011, Springer Science+Business Media, LLC.",
year = "2011",
doi = "10.1007/978-1-60761-974-1_9",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "143--162",
booktitle = "Methods in Molecular Biology",
}