Transgenesis in mouse embryonic stem cells.

Janine van Ree, Wei Zhou, Ming Li, Jan M. van Deursen

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Traditionally, transgenic mice are generated by pronuclear injection of exogenous DNA. This technique has several limitations, including limited control over transgene expression, transgene cytotoxicity, -promiscuity and silencing, and founder mouse sterility. Here we describe two protocols to generate transgenic mice from ES cell clones carrying stably integrated exogenous DNA with inducible transgene expression.

Original languageEnglish (US)
Pages (from-to)143-162
Number of pages20
JournalMethods in molecular biology (Clifton, N.J.)
Volume693
DOIs
StatePublished - 2011

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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