TY - JOUR
T1 - Transgenesis in mouse embryonic stem cells.
AU - van Ree, Janine
AU - Zhou, Wei
AU - Li, Ming
AU - van Deursen, Jan M.
PY - 2011
Y1 - 2011
N2 - Traditionally, transgenic mice are generated by pronuclear injection of exogenous DNA. This technique has several limitations, including limited control over transgene expression, transgene cytotoxicity, -promiscuity and silencing, and founder mouse sterility. Here we describe two protocols to generate transgenic mice from ES cell clones carrying stably integrated exogenous DNA with inducible transgene expression.
AB - Traditionally, transgenic mice are generated by pronuclear injection of exogenous DNA. This technique has several limitations, including limited control over transgene expression, transgene cytotoxicity, -promiscuity and silencing, and founder mouse sterility. Here we describe two protocols to generate transgenic mice from ES cell clones carrying stably integrated exogenous DNA with inducible transgene expression.
UR - http://www.scopus.com/inward/record.url?scp=79952277781&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79952277781&partnerID=8YFLogxK
U2 - 10.1007/978-1-60761-974-1_9
DO - 10.1007/978-1-60761-974-1_9
M3 - Article
C2 - 21080279
AN - SCOPUS:79952277781
VL - 693
SP - 143
EP - 162
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
SN - 1064-3745
ER -