Transgenesis in mouse embryonic stem cells.

Janine van Ree, Wei Zhou, Ming Li, Jan Van Deursen

Research output: Contribution to journal › Article

4 Citations (Scopus)

Abstract

Traditionally, transgenic mice are generated by pronuclear injection of exogenous DNA. This technique has several limitations, including limited control over transgene expression, transgene cytotoxicity, -promiscuity and silencing, and founder mouse sterility. Here we describe two protocols to generate transgenic mice from ES cell clones carrying stably integrated exogenous DNA with inducible transgene expression.

Original languageEnglish (US)
Pages (from-to)143-162
Number of pages20
JournalMethods in molecular biology (Clifton, N.J.)
Volume693
StatePublished - 2011

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Gene Transfer Techniques
Transgenes
Transgenic Mice
DNA
Infertility
Clone Cells
Injections
Mouse Embryonic Stem Cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Transgenesis in mouse embryonic stem cells. / van Ree, Janine; Zhou, Wei; Li, Ming; Van Deursen, Jan.

In: Methods in molecular biology (Clifton, N.J.), Vol. 693, 2011, p. 143-162.

Research output: Contribution to journal › Article

van Ree, J, Zhou, W, Li, M & Van Deursen, J 2011, 'Transgenesis in mouse embryonic stem cells.', Methods in molecular biology (Clifton, N.J.), vol. 693, pp. 143-162.
van Ree J, Zhou W, Li M, Van Deursen J. Transgenesis in mouse embryonic stem cells. Methods in molecular biology (Clifton, N.J.). 2011;693:143-162.
van Ree, Janine ; Zhou, Wei ; Li, Ming ; Van Deursen, Jan. / Transgenesis in mouse embryonic stem cells. In: Methods in molecular biology (Clifton, N.J.). 2011 ; Vol. 693. pp. 143-162.
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