Transgenesis in mouse embryonic stem cells.

Janine van Ree; Wei Zhou; Ming Li; Jan M. van Deursen

doi:10.1007/978-1-60761-974-1_9

Transgenesis in mouse embryonic stem cells.

Janine van Ree, Wei Zhou, Ming Li, Jan M. van Deursen

Pediatric and Adolescent Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Traditionally, transgenic mice are generated by pronuclear injection of exogenous DNA. This technique has several limitations, including limited control over transgene expression, transgene cytotoxicity, -promiscuity and silencing, and founder mouse sterility. Here we describe two protocols to generate transgenic mice from ES cell clones carrying stably integrated exogenous DNA with inducible transgene expression.

Original language	English (US)
Pages (from-to)	143-162
Number of pages	20
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	693
DOIs	https://doi.org/10.1007/978-1-60761-974-1_9
State	Published - 2011

ASJC Scopus subject areas

Molecular Biology
Genetics

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title = "Transgenesis in mouse embryonic stem cells.",

abstract = "Traditionally, transgenic mice are generated by pronuclear injection of exogenous DNA. This technique has several limitations, including limited control over transgene expression, transgene cytotoxicity, -promiscuity and silencing, and founder mouse sterility. Here we describe two protocols to generate transgenic mice from ES cell clones carrying stably integrated exogenous DNA with inducible transgene expression.",

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