Transduction of rabbit saphenous artery: A comparison of naked DNA, liposome complexes, and adenovirus vectors

Kousuke Katsube, Allen Thorp Bishop, Patricia F. Friedrich

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The methods and efficiency of gene transfer into rabbit saphenous artery were examined in this study. The purpose was to develop an animal model capable of evaluating the use of angiogenic gene therapy to revascularize necrotic bone more rapidly and completely than by surgical implantation of blood vessels alone. The success of transduction using adenovirus vectors, liposome/DNA complexes, and naked DNA was evaluated with delivery to both intra-luminal and adventitial sites. Intra-luminal and adventitial (extra-luminal) application was used for the viral and liposome methods. Naked DNA was evaluated only in the intra-luminal site, based upon previous reports. Relative transduction success was expressed as the percentage of total cells with β-galactosidase activity. A 20-mm length of saphenous artery exposed surgically was targeted for lac Z gene transfer. Two days after transduction, the arteries were harvested and stained with X-gal for β-galactosidase activity. The percentage of endothelial, media and adventitial cells with β-galactosidase activity was determined. Intra-arterial injection of adenovirus vector transduced the largest amount of cells in all three areas of the vessel (endothelium, media and adventitia). The adenovirus vectors when applied to the adventitia only transduced adventitial cells. Following intra-arterial injection of liposome/DNA complexes transduction was detected only in endothelium. Extra-luminal liposome and intra-arterial naked DNA delivery resulted in no detectable gene transfer. Intra-arterial delivery of an adenovirus vector would likely provide optimal gene transfer for possible angiogenic gene therapy.

Original languageEnglish (US)
Pages (from-to)1290-1295
Number of pages6
JournalJournal of Orthopaedic Research
Volume22
Issue number6
DOIs
StatePublished - Nov 2004

Fingerprint

Adventitia
Adenoviridae
Liposomes
Arteries
Galactosidases
Rabbits
DNA
Intra-Arterial Injections
Genetic Therapy
Endothelium
Genes
Lac Operon
Blood Vessels
Animal Models
Bone and Bones

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Transduction of rabbit saphenous artery : A comparison of naked DNA, liposome complexes, and adenovirus vectors. / Katsube, Kousuke; Bishop, Allen Thorp; Friedrich, Patricia F.

In: Journal of Orthopaedic Research, Vol. 22, No. 6, 11.2004, p. 1290-1295.

Research output: Contribution to journalArticle

@article{553c1a22cffe402da1285f0b78c7cf41,
title = "Transduction of rabbit saphenous artery: A comparison of naked DNA, liposome complexes, and adenovirus vectors",
abstract = "The methods and efficiency of gene transfer into rabbit saphenous artery were examined in this study. The purpose was to develop an animal model capable of evaluating the use of angiogenic gene therapy to revascularize necrotic bone more rapidly and completely than by surgical implantation of blood vessels alone. The success of transduction using adenovirus vectors, liposome/DNA complexes, and naked DNA was evaluated with delivery to both intra-luminal and adventitial sites. Intra-luminal and adventitial (extra-luminal) application was used for the viral and liposome methods. Naked DNA was evaluated only in the intra-luminal site, based upon previous reports. Relative transduction success was expressed as the percentage of total cells with β-galactosidase activity. A 20-mm length of saphenous artery exposed surgically was targeted for lac Z gene transfer. Two days after transduction, the arteries were harvested and stained with X-gal for β-galactosidase activity. The percentage of endothelial, media and adventitial cells with β-galactosidase activity was determined. Intra-arterial injection of adenovirus vector transduced the largest amount of cells in all three areas of the vessel (endothelium, media and adventitia). The adenovirus vectors when applied to the adventitia only transduced adventitial cells. Following intra-arterial injection of liposome/DNA complexes transduction was detected only in endothelium. Extra-luminal liposome and intra-arterial naked DNA delivery resulted in no detectable gene transfer. Intra-arterial delivery of an adenovirus vector would likely provide optimal gene transfer for possible angiogenic gene therapy.",
author = "Kousuke Katsube and Bishop, {Allen Thorp} and Friedrich, {Patricia F.}",
year = "2004",
month = "11",
doi = "10.1016/j.orthres.2004.05.005",
language = "English (US)",
volume = "22",
pages = "1290--1295",
journal = "Journal of Orthopaedic Research",
issn = "0736-0266",
publisher = "John Wiley and Sons Inc.",
number = "6",

}

TY - JOUR

T1 - Transduction of rabbit saphenous artery

T2 - A comparison of naked DNA, liposome complexes, and adenovirus vectors

AU - Katsube, Kousuke

AU - Bishop, Allen Thorp

AU - Friedrich, Patricia F.

PY - 2004/11

Y1 - 2004/11

N2 - The methods and efficiency of gene transfer into rabbit saphenous artery were examined in this study. The purpose was to develop an animal model capable of evaluating the use of angiogenic gene therapy to revascularize necrotic bone more rapidly and completely than by surgical implantation of blood vessels alone. The success of transduction using adenovirus vectors, liposome/DNA complexes, and naked DNA was evaluated with delivery to both intra-luminal and adventitial sites. Intra-luminal and adventitial (extra-luminal) application was used for the viral and liposome methods. Naked DNA was evaluated only in the intra-luminal site, based upon previous reports. Relative transduction success was expressed as the percentage of total cells with β-galactosidase activity. A 20-mm length of saphenous artery exposed surgically was targeted for lac Z gene transfer. Two days after transduction, the arteries were harvested and stained with X-gal for β-galactosidase activity. The percentage of endothelial, media and adventitial cells with β-galactosidase activity was determined. Intra-arterial injection of adenovirus vector transduced the largest amount of cells in all three areas of the vessel (endothelium, media and adventitia). The adenovirus vectors when applied to the adventitia only transduced adventitial cells. Following intra-arterial injection of liposome/DNA complexes transduction was detected only in endothelium. Extra-luminal liposome and intra-arterial naked DNA delivery resulted in no detectable gene transfer. Intra-arterial delivery of an adenovirus vector would likely provide optimal gene transfer for possible angiogenic gene therapy.

AB - The methods and efficiency of gene transfer into rabbit saphenous artery were examined in this study. The purpose was to develop an animal model capable of evaluating the use of angiogenic gene therapy to revascularize necrotic bone more rapidly and completely than by surgical implantation of blood vessels alone. The success of transduction using adenovirus vectors, liposome/DNA complexes, and naked DNA was evaluated with delivery to both intra-luminal and adventitial sites. Intra-luminal and adventitial (extra-luminal) application was used for the viral and liposome methods. Naked DNA was evaluated only in the intra-luminal site, based upon previous reports. Relative transduction success was expressed as the percentage of total cells with β-galactosidase activity. A 20-mm length of saphenous artery exposed surgically was targeted for lac Z gene transfer. Two days after transduction, the arteries were harvested and stained with X-gal for β-galactosidase activity. The percentage of endothelial, media and adventitial cells with β-galactosidase activity was determined. Intra-arterial injection of adenovirus vector transduced the largest amount of cells in all three areas of the vessel (endothelium, media and adventitia). The adenovirus vectors when applied to the adventitia only transduced adventitial cells. Following intra-arterial injection of liposome/DNA complexes transduction was detected only in endothelium. Extra-luminal liposome and intra-arterial naked DNA delivery resulted in no detectable gene transfer. Intra-arterial delivery of an adenovirus vector would likely provide optimal gene transfer for possible angiogenic gene therapy.

UR - http://www.scopus.com/inward/record.url?scp=4844224147&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4844224147&partnerID=8YFLogxK

U2 - 10.1016/j.orthres.2004.05.005

DO - 10.1016/j.orthres.2004.05.005

M3 - Article

C2 - 15475211

AN - SCOPUS:4844224147

VL - 22

SP - 1290

EP - 1295

JO - Journal of Orthopaedic Research

JF - Journal of Orthopaedic Research

SN - 0736-0266

IS - 6

ER -