Transduction of compressive stress by bronchial epithelium

Daniel J Tschumperlin, J. M. Drazen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The epithelial lining of the asthmatic airway is exposed to compressive stress as a consequence of smooth muscle constriction. We have shown previously that in vitro compression of bronchial epithelial cells stimulates extracellular signal-regulated kinase (ERK) phosphorylation and downstream gene expression. Here we show that inhibition of signaling through the epidermal growth factor receptor (EGFR) with a tyrosine kinase inhibitor (AG1478) or a neutralizing antibody to the ligand-binding domain of the EGFR blocks compression-induced ERK phosphorylation. A metalloprotease inhibitor (Galardin) and a neutralizing antibody to heparin binding epidermal growth factor (HB-EGF), but not EGF, also attenuates the compression-induced ERK activation. Our results demonstrate that compressive activation of the ERK signaling pathway requires signaling through the EGFR, and involves metalloprotease-dependent shedding of HB-EGF.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages641-642
Number of pages2
Volume1
StatePublished - 2002
Externally publishedYes
EventProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States
Duration: Oct 23 2002Oct 26 2002

Other

OtherProceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS)
CountryUnited States
CityHouston, TX
Period10/23/0210/26/02

Fingerprint

Extracellular Signal-Regulated MAP Kinases
Compressive stress
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Phosphorylation
Metalloproteases
Neutralizing Antibodies
Heparin
Chemical activation
Antibodies
Linings
Gene expression
Protein-Tyrosine Kinases
Muscle
Ligands

Keywords

  • EGF receptor
  • MAP kinase
  • Mechanotransduction

ASJC Scopus subject areas

  • Bioengineering

Cite this

Tschumperlin, D. J., & Drazen, J. M. (2002). Transduction of compressive stress by bronchial epithelium. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 1, pp. 641-642)

Transduction of compressive stress by bronchial epithelium. / Tschumperlin, Daniel J; Drazen, J. M.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 2002. p. 641-642.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tschumperlin, DJ & Drazen, JM 2002, Transduction of compressive stress by bronchial epithelium. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 1, pp. 641-642, Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS), Houston, TX, United States, 10/23/02.
Tschumperlin DJ, Drazen JM. Transduction of compressive stress by bronchial epithelium. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1. 2002. p. 641-642
Tschumperlin, Daniel J ; Drazen, J. M. / Transduction of compressive stress by bronchial epithelium. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 2002. pp. 641-642
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