Abstract
Background Human saphenous vein (HSV) is the autologous conduit of choice for peripheral vascular reconstruction. Injury during harvest leads to vasospasm and a thrombogenic endoluminal surface. A proteomic transduction approach was developed to prevent vein graft vasospasm and thrombosis. Methods Recombinant HSP20 protein linked to the TAT protein transduction domain was generated in a bacterial expression system (TAT-HSP20). The effect of this protein on the inhibition of smooth muscle contraction was determined using rings of rabbit aorta and HSV in a muscle bath. In addition, the effects of TAT-HSP20 on platelet aggregation were determined in vitro using human citrated whole blood. Results Recombinant TAT-HSP20 inhibited norepinephrine-induced contraction of rabbit aortic and HSV segments. Similarly, TAT-HSP20 induced smooth muscle relaxation in HSV segments precontracted with norepinephrine. In human-citrated whole blood, platelet aggregation was significantly inhibited by TAT-HSP20 in a dose-dependent manner. Conclusions The results of this study demonstrate that recombinant TAT-HSP20 inhibits vascular smooth muscle contraction and platelet aggregation. This suggests that HSP20 may be an ideal effector molecule to target as a proteomic approach to enhance early vein graft patency rates by preventing acute vasospasm and thrombosis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 573-578 |
| Number of pages | 6 |
| Journal | Surgery |
| Volume | 136 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 2004 |
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ASJC Scopus subject areas
- Surgery
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Transducible recombinant small heat shock-related protein, HSP20, inhibits vasospasm and platelet aggregation. / McLemore, Elisabeth C.; Tessier, Deron J.; Robert Flynn, C.; Furnish, Elizabeth J.; Komalavilas, Padmini; Thresher, Jeffrey S.; Joshi, Lokesh; Stone, William M.; Fowl, Richard J.; Brophy, Colleen M.
In: Surgery, Vol. 136, No. 3, 09.2004, p. 573-578.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transducible recombinant small heat shock-related protein, HSP20, inhibits vasospasm and platelet aggregation
AU - McLemore, Elisabeth C.
AU - Tessier, Deron J.
AU - Robert Flynn, C.
AU - Furnish, Elizabeth J.
AU - Komalavilas, Padmini
AU - Thresher, Jeffrey S.
AU - Joshi, Lokesh
AU - Stone, William M.
AU - Fowl, Richard J.
AU - Brophy, Colleen M.
PY - 2004/9
Y1 - 2004/9
N2 - Background Human saphenous vein (HSV) is the autologous conduit of choice for peripheral vascular reconstruction. Injury during harvest leads to vasospasm and a thrombogenic endoluminal surface. A proteomic transduction approach was developed to prevent vein graft vasospasm and thrombosis. Methods Recombinant HSP20 protein linked to the TAT protein transduction domain was generated in a bacterial expression system (TAT-HSP20). The effect of this protein on the inhibition of smooth muscle contraction was determined using rings of rabbit aorta and HSV in a muscle bath. In addition, the effects of TAT-HSP20 on platelet aggregation were determined in vitro using human citrated whole blood. Results Recombinant TAT-HSP20 inhibited norepinephrine-induced contraction of rabbit aortic and HSV segments. Similarly, TAT-HSP20 induced smooth muscle relaxation in HSV segments precontracted with norepinephrine. In human-citrated whole blood, platelet aggregation was significantly inhibited by TAT-HSP20 in a dose-dependent manner. Conclusions The results of this study demonstrate that recombinant TAT-HSP20 inhibits vascular smooth muscle contraction and platelet aggregation. This suggests that HSP20 may be an ideal effector molecule to target as a proteomic approach to enhance early vein graft patency rates by preventing acute vasospasm and thrombosis.
AB - Background Human saphenous vein (HSV) is the autologous conduit of choice for peripheral vascular reconstruction. Injury during harvest leads to vasospasm and a thrombogenic endoluminal surface. A proteomic transduction approach was developed to prevent vein graft vasospasm and thrombosis. Methods Recombinant HSP20 protein linked to the TAT protein transduction domain was generated in a bacterial expression system (TAT-HSP20). The effect of this protein on the inhibition of smooth muscle contraction was determined using rings of rabbit aorta and HSV in a muscle bath. In addition, the effects of TAT-HSP20 on platelet aggregation were determined in vitro using human citrated whole blood. Results Recombinant TAT-HSP20 inhibited norepinephrine-induced contraction of rabbit aortic and HSV segments. Similarly, TAT-HSP20 induced smooth muscle relaxation in HSV segments precontracted with norepinephrine. In human-citrated whole blood, platelet aggregation was significantly inhibited by TAT-HSP20 in a dose-dependent manner. Conclusions The results of this study demonstrate that recombinant TAT-HSP20 inhibits vascular smooth muscle contraction and platelet aggregation. This suggests that HSP20 may be an ideal effector molecule to target as a proteomic approach to enhance early vein graft patency rates by preventing acute vasospasm and thrombosis.
UR - http://www.scopus.com/inward/record.url?scp=4444272910&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4444272910&partnerID=8YFLogxK
U2 - 10.1016/j.surg.2004.04.024
DO - 10.1016/j.surg.2004.04.024
M3 - Article
C2 - 15349104
AN - SCOPUS:4444272910
VL - 136
SP - 573
EP - 578
JO - Surgery (United States)
JF - Surgery (United States)
SN - 0039-6060
IS - 3
ER -