Transcriptomic and Immunohistochemical Profiling of SLC6A14 in Pancreatic Ductal Adenocarcinoma

Alan R. Penheiter; Sibel Erdogan; Stephen J. Murphy; Steven N. Hart; Joema Felipe Lima; Fariborz Rakhshan Rohakhtar; Daniel R. O'Brien; William R. Bamlet; Ryan E. Wuertz; Thomas C. Smyrk; Fergus J. Couch; George Vasmatzis; Claire E. Bender; Stephanie K. Carlson

doi:10.1155/2015/593572

Transcriptomic and Immunohistochemical Profiling of SLC6A14 in Pancreatic Ductal Adenocarcinoma

Alan R. Penheiter, Sibel Erdogan, Stephen J. Murphy, Steven N. Hart, Joema Felipe Lima, Fariborz Rakhshan Rohakhtar, Daniel R. O'Brien, William R. Bamlet, Ryan E. Wuertz, Thomas C. Smyrk, Fergus J. Couch, George Vasmatzis, Claire E. Bender, Stephanie K. Carlson

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17 Scopus citations

Abstract

We used a target-centric strategy to identify transporter proteins upregulated in pancreatic ductal adenocarcinoma (PDAC) as potential targets for a functional imaging probe to complement existing anatomical imaging approaches. We performed transcriptomic profiling (microarray and RNASeq) on histologically confirmed primary PDAC tumors and normal pancreas tissue from 33 patients, including five patients whose tumors were not visible on computed tomography. Target expression was confirmed with immunohistochemistry on tissue microarrays from 94 PDAC patients. The best imaging target identified was SLC6A14 (a neutral and basic amino acid transporter). SLC6A14 was overexpressed at the transcriptional level in all patients and expressed at the protein level in 95% of PDAC tumors. Very little is known about the role of SLC6A14 in PDAC and our results demonstrate that this target merits further investigation as a candidate transporter for functional imaging of PDAC.

Original language	English (US)
Article number	593572
Journal	BioMed Research International
Volume	2015
DOIs	https://doi.org/10.1155/2015/593572
State	Published - 2015

ASJC Scopus subject areas

General Immunology and Microbiology
General Biochemistry, Genetics and Molecular Biology

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Penheiter, A. R., Erdogan, S., Murphy, S. J., Hart, S. N., Felipe Lima, J., Rakhshan Rohakhtar, F., O'Brien, D. R., Bamlet, W. R., Wuertz, R. E., Smyrk, T. C., Couch, F. J., Vasmatzis, G., Bender, C. E., & Carlson, S. K. (2015). Transcriptomic and Immunohistochemical Profiling of SLC6A14 in Pancreatic Ductal Adenocarcinoma. BioMed Research International, 2015, Article 593572. https://doi.org/10.1155/2015/593572

Penheiter, AR, Erdogan, S, Murphy, SJ , Hart, SN, Felipe Lima, J, Rakhshan Rohakhtar, F, O'Brien, DR, Bamlet, WR, Wuertz, RE, Smyrk, TC, Couch, FJ , Vasmatzis, G, Bender, CE & Carlson, SK 2015, 'Transcriptomic and Immunohistochemical Profiling of SLC6A14 in Pancreatic Ductal Adenocarcinoma', BioMed Research International, vol. 2015, 593572. https://doi.org/10.1155/2015/593572

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title = "Transcriptomic and Immunohistochemical Profiling of SLC6A14 in Pancreatic Ductal Adenocarcinoma",

abstract = "We used a target-centric strategy to identify transporter proteins upregulated in pancreatic ductal adenocarcinoma (PDAC) as potential targets for a functional imaging probe to complement existing anatomical imaging approaches. We performed transcriptomic profiling (microarray and RNASeq) on histologically confirmed primary PDAC tumors and normal pancreas tissue from 33 patients, including five patients whose tumors were not visible on computed tomography. Target expression was confirmed with immunohistochemistry on tissue microarrays from 94 PDAC patients. The best imaging target identified was SLC6A14 (a neutral and basic amino acid transporter). SLC6A14 was overexpressed at the transcriptional level in all patients and expressed at the protein level in 95% of PDAC tumors. Very little is known about the role of SLC6A14 in PDAC and our results demonstrate that this target merits further investigation as a candidate transporter for functional imaging of PDAC.",

author = "Penheiter, {Alan R.} and Sibel Erdogan and Murphy, {Stephen J.} and Hart, {Steven N.} and {Felipe Lima}, Joema and {Rakhshan Rohakhtar}, Fariborz and O'Brien, {Daniel R.} and Bamlet, {William R.} and Wuertz, {Ryan E.} and Smyrk, {Thomas C.} and Couch, {Fergus J.} and George Vasmatzis and Bender, {Claire E.} and Carlson, {Stephanie K.}",

note = "Publisher Copyright: {\textcopyright} 2015 Alan R. Penheiter et al.",

year = "2015",

doi = "10.1155/2015/593572",

language = "English (US)",

volume = "2015",

journal = "BioMed Research International",

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AU - Penheiter, Alan R.

AU - Erdogan, Sibel

AU - Murphy, Stephen J.

AU - Hart, Steven N.

AU - Felipe Lima, Joema

AU - Rakhshan Rohakhtar, Fariborz

AU - O'Brien, Daniel R.

AU - Bamlet, William R.

AU - Wuertz, Ryan E.

AU - Smyrk, Thomas C.

AU - Couch, Fergus J.

AU - Vasmatzis, George

AU - Bender, Claire E.

AU - Carlson, Stephanie K.

PY - 2015

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N2 - We used a target-centric strategy to identify transporter proteins upregulated in pancreatic ductal adenocarcinoma (PDAC) as potential targets for a functional imaging probe to complement existing anatomical imaging approaches. We performed transcriptomic profiling (microarray and RNASeq) on histologically confirmed primary PDAC tumors and normal pancreas tissue from 33 patients, including five patients whose tumors were not visible on computed tomography. Target expression was confirmed with immunohistochemistry on tissue microarrays from 94 PDAC patients. The best imaging target identified was SLC6A14 (a neutral and basic amino acid transporter). SLC6A14 was overexpressed at the transcriptional level in all patients and expressed at the protein level in 95% of PDAC tumors. Very little is known about the role of SLC6A14 in PDAC and our results demonstrate that this target merits further investigation as a candidate transporter for functional imaging of PDAC.

AB - We used a target-centric strategy to identify transporter proteins upregulated in pancreatic ductal adenocarcinoma (PDAC) as potential targets for a functional imaging probe to complement existing anatomical imaging approaches. We performed transcriptomic profiling (microarray and RNASeq) on histologically confirmed primary PDAC tumors and normal pancreas tissue from 33 patients, including five patients whose tumors were not visible on computed tomography. Target expression was confirmed with immunohistochemistry on tissue microarrays from 94 PDAC patients. The best imaging target identified was SLC6A14 (a neutral and basic amino acid transporter). SLC6A14 was overexpressed at the transcriptional level in all patients and expressed at the protein level in 95% of PDAC tumors. Very little is known about the role of SLC6A14 in PDAC and our results demonstrate that this target merits further investigation as a candidate transporter for functional imaging of PDAC.

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Transcriptomic and Immunohistochemical Profiling of SLC6A14 in Pancreatic Ductal Adenocarcinoma

Abstract

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