Transcriptome analysis of garlic-induced hepatoprotection against alcoholic fatty liver

Rajasekaran Raghu, Chun Ting Liu, Mong Hsun Tsai, Xiaojia Tang, Krishna R. Kalari, Subbaya Subramanian, Lee Yan Sheen

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Fatty liver induced by alcohol abuse is a major worldwide health hazard leading to morbidity and mortality. Previous studies indicate antifatty liver properties of garlic. This study investigated the molecular mechanisms of garlic oil (GO) or diallyl disulfide (DADS) imparted hepatoprotection against alcohol induced fatty liver in C57BL/6 mice using microarray-based global gene expression analysis. Alcohol liquid diet resulted in severe fatty liver with increased levels of serum aspartate aminotransferease and alanine aminotransferease as well as triglycerides and decreased levels of liver glutathione and antioxidant enzymes. The major canonical pathways implicated by alcohol treatment are the metabolisms of xenobiotics by cytochrome P450, glutathione, and arachidonic acid. Treatment with DADS or GO normalized the serum aminotransferease levels and liver antioxidant enzymes and reduced the contents of triglycerides and cholesterol. The canonical pathways involved in the amelioration of liver include arachidonic acid metabolism, altered T cell and B cell signaling, tryptophan metabolism, antigen presentation pathway for DADS, metabolism of xenobiotics, mitotic roles of Polo-like kinase, fatty acid metabolism, LPS/IL-1 mediated inhibition of RXR function, and C21-steroid hormone metabolism for GO.

Original languageEnglish (US)
Pages (from-to)11104-11119
Number of pages16
JournalJournal of Agricultural and Food Chemistry
Volume60
Issue number44
DOIs
StatePublished - Nov 7 2012

Keywords

  • antifatty liver
  • diallyl disulfide
  • garlic oil
  • mechanism
  • transcriptome

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Fingerprint Dive into the research topics of 'Transcriptome analysis of garlic-induced hepatoprotection against alcoholic fatty liver'. Together they form a unique fingerprint.

  • Cite this