Transcriptional silencing of zinc finger protein 185 identified by expression profiling is associated with prostate cancer progression

Donkena Krishna Vanaja, John C. Cheville, Steve J. Iturria, Charles Y F Young

Research output: Contribution to journalArticle

228 Citations (Scopus)

Abstract

We profiled the expression of genes in benign and untreated human prostate cancer tissues using oligonucleotide microarrays. We report here 50 genes with distinct expression patterns in metastatic and confined tumors (Gleason score 6 and 9; lymph node invasive and noninvasive). Validation of expression profiles of 6 genes by quantitative PCR revealed a strong inverse correlation in the expression of zinc finger protein 185 (ZNF185), bullous pemphigoid antigen gene (BPAG1), and prostate secretory protein (PSP94) with progression of prostate cancer. Treatment of prostate cancer cell lines with 5-aza-2′-deoxycytidine (5-Aza-CdR), an inhibitor of DNA methylation, restored ZNF185 expression levels. Moreover, methylation-specific PCR confirmed methylation of the 5′CpG islands of the ZNF185 gene in all of the metastatic tissues and 44% of the localized tumor tissues, as well as in the prostate cancer cell lines tested. Thus, transcriptional silencing of ZNF185 by methylation in prostate tumor tissues implicates the ZNF185 gene in prostate tumorigenesis.

Original languageEnglish (US)
Pages (from-to)3877-3882
Number of pages6
JournalCancer Research
Volume63
Issue number14
StatePublished - Jul 15 2003

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Zinc Fingers
Prostatic Neoplasms
Methylation
decitabine
Proteins
Prostate
Cell Line
Bullous Pemphigoid
Neoplasms
Polymerase Chain Reaction
Neoplasm Grading
DNA Methylation
Oligonucleotide Array Sequence Analysis
Transcriptome
Islands
Genes
Carcinogenesis
Lymph Nodes
Gene Expression
Antigens

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Transcriptional silencing of zinc finger protein 185 identified by expression profiling is associated with prostate cancer progression. / Vanaja, Donkena Krishna; Cheville, John C.; Iturria, Steve J.; Young, Charles Y F.

In: Cancer Research, Vol. 63, No. 14, 15.07.2003, p. 3877-3882.

Research output: Contribution to journalArticle

Vanaja, Donkena Krishna ; Cheville, John C. ; Iturria, Steve J. ; Young, Charles Y F. / Transcriptional silencing of zinc finger protein 185 identified by expression profiling is associated with prostate cancer progression. In: Cancer Research. 2003 ; Vol. 63, No. 14. pp. 3877-3882.
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