Transcriptional signatures of influenza A/H1N1-specific IgG memory-like B cell response in older individuals

Iana H. Haralambieva, Inna G. Ovsyannikova, Richard B Kennedy, Michael T. Zimmermann, Diane E. Grill, Ann L Oberg, Gregory A. Poland

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Background Studies suggest that the recall-based humoral immune responses to influenza A/H1N1 originates from activated memory B cells. The aim of this study was to identify baseline, early and late blood transcriptional signatures (in peripheral blood mononuclear cells/PBMCs) associated with memory B cell response following influenza vaccination. Methods We used pre- and post-vaccination mRNA-Seq transcriptional profiling on samples from 159 subjects (50–74 years old) following receipt of seasonal trivalent influenza vaccine containing the A/California/7/2009/H1N1-like virus, and penalized regression modeling to identify associations with influenza A/H1N1-specific memory B cell ELISPOT response after vaccination. Results Genesets and genes (p-value range 7.92E−08 to 0.00018, q-value range 0.00019–0.039) demonstrating significant associations (of gene expression levels) with memory B cell response suggest the importance of metabolic (cholesterol and lipid metabolism-related), cell migration/adhesion, MAP kinase, NF-kB cell signaling (chemokine/cytokine signaling) and transcriptional regulation gene signatures in the development of memory B cell response after influenza vaccination. Conclusion Through an unbiased transcriptome-wide profiling approach, our study identified signatures of memory B cell response following influenza vaccination, highlighting the underappreciated role of metabolic changes (among the other immune function-related events) in the regulation of influenza vaccine-induced immune memory.

Original languageEnglish (US)
Pages (from-to)3993-4002
Number of pages10
JournalVaccine
Volume34
Issue number34
DOIs
StatePublished - Jul 25 2016

Keywords

  • Aging
  • B-lymphocytes
  • Enzyme-linked immunospot assay
  • Gene expression
  • Immunity, Humoral
  • Influenza A virus, H1N1 subtype
  • Influenza vaccines
  • Influenza, Human
  • RNA
  • Sequence analysis
  • Vaccination

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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