Transcriptional signatures of influenza A/H1N1-specific IgG memory-like B cell response in older individuals

Iana H. Haralambieva, Inna G. Ovsyannikova, Richard B Kennedy, Michael T. Zimmermann, Diane E. Grill, Ann L Oberg, Gregory A. Poland

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background Studies suggest that the recall-based humoral immune responses to influenza A/H1N1 originates from activated memory B cells. The aim of this study was to identify baseline, early and late blood transcriptional signatures (in peripheral blood mononuclear cells/PBMCs) associated with memory B cell response following influenza vaccination. Methods We used pre- and post-vaccination mRNA-Seq transcriptional profiling on samples from 159 subjects (50–74 years old) following receipt of seasonal trivalent influenza vaccine containing the A/California/7/2009/H1N1-like virus, and penalized regression modeling to identify associations with influenza A/H1N1-specific memory B cell ELISPOT response after vaccination. Results Genesets and genes (p-value range 7.92E−08 to 0.00018, q-value range 0.00019–0.039) demonstrating significant associations (of gene expression levels) with memory B cell response suggest the importance of metabolic (cholesterol and lipid metabolism-related), cell migration/adhesion, MAP kinase, NF-kB cell signaling (chemokine/cytokine signaling) and transcriptional regulation gene signatures in the development of memory B cell response after influenza vaccination. Conclusion Through an unbiased transcriptome-wide profiling approach, our study identified signatures of memory B cell response following influenza vaccination, highlighting the underappreciated role of metabolic changes (among the other immune function-related events) in the regulation of influenza vaccine-induced immune memory.

Original languageEnglish (US)
Pages (from-to)3993-4002
Number of pages10
JournalVaccine
Volume34
Issue number34
DOIs
StatePublished - Jul 25 2016

Fingerprint

influenza
Human Influenza
B-lymphocytes
Vaccination
B-Lymphocytes
Immunoglobulin G
vaccination
Influenza Vaccines
mononuclear leukocytes
Enzyme-Linked Immunospot Assay
H1N1 Subtype Influenza A Virus
vaccines
NF-kappa B
transcription factor NF-kappa B
Gene Expression Profiling
cholesterol metabolism
Humoral Immunity
Lipid Metabolism
Chemokines
Cell Adhesion

Keywords

  • Aging
  • B-lymphocytes
  • Enzyme-linked immunospot assay
  • Gene expression
  • Immunity, Humoral
  • Influenza A virus, H1N1 subtype
  • Influenza vaccines
  • Influenza, Human
  • RNA
  • Sequence analysis
  • Vaccination

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Transcriptional signatures of influenza A/H1N1-specific IgG memory-like B cell response in older individuals. / Haralambieva, Iana H.; Ovsyannikova, Inna G.; Kennedy, Richard B; Zimmermann, Michael T.; Grill, Diane E.; Oberg, Ann L; Poland, Gregory A.

In: Vaccine, Vol. 34, No. 34, 25.07.2016, p. 3993-4002.

Research output: Contribution to journalArticle

Haralambieva, Iana H. ; Ovsyannikova, Inna G. ; Kennedy, Richard B ; Zimmermann, Michael T. ; Grill, Diane E. ; Oberg, Ann L ; Poland, Gregory A. / Transcriptional signatures of influenza A/H1N1-specific IgG memory-like B cell response in older individuals. In: Vaccine. 2016 ; Vol. 34, No. 34. pp. 3993-4002.
@article{916485c07b324a62b89ccc4946127261,
title = "Transcriptional signatures of influenza A/H1N1-specific IgG memory-like B cell response in older individuals",
abstract = "Background Studies suggest that the recall-based humoral immune responses to influenza A/H1N1 originates from activated memory B cells. The aim of this study was to identify baseline, early and late blood transcriptional signatures (in peripheral blood mononuclear cells/PBMCs) associated with memory B cell response following influenza vaccination. Methods We used pre- and post-vaccination mRNA-Seq transcriptional profiling on samples from 159 subjects (50–74 years old) following receipt of seasonal trivalent influenza vaccine containing the A/California/7/2009/H1N1-like virus, and penalized regression modeling to identify associations with influenza A/H1N1-specific memory B cell ELISPOT response after vaccination. Results Genesets and genes (p-value range 7.92E−08 to 0.00018, q-value range 0.00019–0.039) demonstrating significant associations (of gene expression levels) with memory B cell response suggest the importance of metabolic (cholesterol and lipid metabolism-related), cell migration/adhesion, MAP kinase, NF-kB cell signaling (chemokine/cytokine signaling) and transcriptional regulation gene signatures in the development of memory B cell response after influenza vaccination. Conclusion Through an unbiased transcriptome-wide profiling approach, our study identified signatures of memory B cell response following influenza vaccination, highlighting the underappreciated role of metabolic changes (among the other immune function-related events) in the regulation of influenza vaccine-induced immune memory.",
keywords = "Aging, B-lymphocytes, Enzyme-linked immunospot assay, Gene expression, Immunity, Humoral, Influenza A virus, H1N1 subtype, Influenza vaccines, Influenza, Human, RNA, Sequence analysis, Vaccination",
author = "Haralambieva, {Iana H.} and Ovsyannikova, {Inna G.} and Kennedy, {Richard B} and Zimmermann, {Michael T.} and Grill, {Diane E.} and Oberg, {Ann L} and Poland, {Gregory A.}",
year = "2016",
month = "7",
day = "25",
doi = "10.1016/j.vaccine.2016.06.034",
language = "English (US)",
volume = "34",
pages = "3993--4002",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "34",

}

TY - JOUR

T1 - Transcriptional signatures of influenza A/H1N1-specific IgG memory-like B cell response in older individuals

AU - Haralambieva, Iana H.

AU - Ovsyannikova, Inna G.

AU - Kennedy, Richard B

AU - Zimmermann, Michael T.

AU - Grill, Diane E.

AU - Oberg, Ann L

AU - Poland, Gregory A.

PY - 2016/7/25

Y1 - 2016/7/25

N2 - Background Studies suggest that the recall-based humoral immune responses to influenza A/H1N1 originates from activated memory B cells. The aim of this study was to identify baseline, early and late blood transcriptional signatures (in peripheral blood mononuclear cells/PBMCs) associated with memory B cell response following influenza vaccination. Methods We used pre- and post-vaccination mRNA-Seq transcriptional profiling on samples from 159 subjects (50–74 years old) following receipt of seasonal trivalent influenza vaccine containing the A/California/7/2009/H1N1-like virus, and penalized regression modeling to identify associations with influenza A/H1N1-specific memory B cell ELISPOT response after vaccination. Results Genesets and genes (p-value range 7.92E−08 to 0.00018, q-value range 0.00019–0.039) demonstrating significant associations (of gene expression levels) with memory B cell response suggest the importance of metabolic (cholesterol and lipid metabolism-related), cell migration/adhesion, MAP kinase, NF-kB cell signaling (chemokine/cytokine signaling) and transcriptional regulation gene signatures in the development of memory B cell response after influenza vaccination. Conclusion Through an unbiased transcriptome-wide profiling approach, our study identified signatures of memory B cell response following influenza vaccination, highlighting the underappreciated role of metabolic changes (among the other immune function-related events) in the regulation of influenza vaccine-induced immune memory.

AB - Background Studies suggest that the recall-based humoral immune responses to influenza A/H1N1 originates from activated memory B cells. The aim of this study was to identify baseline, early and late blood transcriptional signatures (in peripheral blood mononuclear cells/PBMCs) associated with memory B cell response following influenza vaccination. Methods We used pre- and post-vaccination mRNA-Seq transcriptional profiling on samples from 159 subjects (50–74 years old) following receipt of seasonal trivalent influenza vaccine containing the A/California/7/2009/H1N1-like virus, and penalized regression modeling to identify associations with influenza A/H1N1-specific memory B cell ELISPOT response after vaccination. Results Genesets and genes (p-value range 7.92E−08 to 0.00018, q-value range 0.00019–0.039) demonstrating significant associations (of gene expression levels) with memory B cell response suggest the importance of metabolic (cholesterol and lipid metabolism-related), cell migration/adhesion, MAP kinase, NF-kB cell signaling (chemokine/cytokine signaling) and transcriptional regulation gene signatures in the development of memory B cell response after influenza vaccination. Conclusion Through an unbiased transcriptome-wide profiling approach, our study identified signatures of memory B cell response following influenza vaccination, highlighting the underappreciated role of metabolic changes (among the other immune function-related events) in the regulation of influenza vaccine-induced immune memory.

KW - Aging

KW - B-lymphocytes

KW - Enzyme-linked immunospot assay

KW - Gene expression

KW - Immunity, Humoral

KW - Influenza A virus, H1N1 subtype

KW - Influenza vaccines

KW - Influenza, Human

KW - RNA

KW - Sequence analysis

KW - Vaccination

UR - http://www.scopus.com/inward/record.url?scp=84979279998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84979279998&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2016.06.034

DO - 10.1016/j.vaccine.2016.06.034

M3 - Article

C2 - 27317456

AN - SCOPUS:84979279998

VL - 34

SP - 3993

EP - 4002

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 34

ER -