Abstract
Objective: To compare full transcriptome expression levels of matched tumor and normal samples from patients with oropharyngeal carcinoma stratified by known tumor etiologic factors. Patients and Methods: Full transcriptome sequencing was analyzed for 10 matched tumor and normal tissue samples from patients with previously untreated oropharyngeal carcinoma. Transcriptomes were analyzed using massively parallel messenger RNA sequencing and validated using the NanoString nCounter system. Global gene expression levels were compared in samples grouped by smoking status and human papillomavirus status. This study was completed between June 10, 2010, and June 30, 2011. Results: Global gene expression analysis indicated tumor tissue from former smokers grouped more closely to the never smokers than the current smokers. Pathway analysis revealed alterations in the expression of genes involved in the p53 DNA damage-repair pathway, including CHEK2 and ATR, which display patterns of increased expression that is associated with human papillomavirus-negative current smokers rather than former or never smokers. Conclusion: These findings support the application of messenger RNA sequencing technology as an important clinical tool for more accurately stratifying patients based on individual tumor biology with the goal of improving our understanding of tumor prognosis and treatment response, ultimately leading to individualized patient care strategies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 226-232 |
| Number of pages | 7 |
| Journal | Mayo Clinic Proceedings |
| Volume | 87 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2012 |
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ASJC Scopus subject areas
- Medicine(all)
Cite this
Transcriptional profiling by sequencing of oropharyngeal cancer. / Laborde, Rebecca R.; Wang, Vivian W.; Smith, Todd M.; Olson, N. Eric; Olsen, Steven M.; García, Joaquín J.; Olsen, Kerry D.; Moore, Eric J.; Kasperbauer, Jan; Tombers, Nicole M.; Smith, David I.
In: Mayo Clinic Proceedings, Vol. 87, No. 3, 2012, p. 226-232.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transcriptional profiling by sequencing of oropharyngeal cancer
AU - Laborde, Rebecca R.
AU - Wang, Vivian W.
AU - Smith, Todd M.
AU - Olson, N. Eric
AU - Olsen, Steven M.
AU - García, Joaquín J.
AU - Olsen, Kerry D.
AU - Moore, Eric J.
AU - Kasperbauer, Jan
AU - Tombers, Nicole M.
AU - Smith, David I
PY - 2012
Y1 - 2012
N2 - Objective: To compare full transcriptome expression levels of matched tumor and normal samples from patients with oropharyngeal carcinoma stratified by known tumor etiologic factors. Patients and Methods: Full transcriptome sequencing was analyzed for 10 matched tumor and normal tissue samples from patients with previously untreated oropharyngeal carcinoma. Transcriptomes were analyzed using massively parallel messenger RNA sequencing and validated using the NanoString nCounter system. Global gene expression levels were compared in samples grouped by smoking status and human papillomavirus status. This study was completed between June 10, 2010, and June 30, 2011. Results: Global gene expression analysis indicated tumor tissue from former smokers grouped more closely to the never smokers than the current smokers. Pathway analysis revealed alterations in the expression of genes involved in the p53 DNA damage-repair pathway, including CHEK2 and ATR, which display patterns of increased expression that is associated with human papillomavirus-negative current smokers rather than former or never smokers. Conclusion: These findings support the application of messenger RNA sequencing technology as an important clinical tool for more accurately stratifying patients based on individual tumor biology with the goal of improving our understanding of tumor prognosis and treatment response, ultimately leading to individualized patient care strategies.
AB - Objective: To compare full transcriptome expression levels of matched tumor and normal samples from patients with oropharyngeal carcinoma stratified by known tumor etiologic factors. Patients and Methods: Full transcriptome sequencing was analyzed for 10 matched tumor and normal tissue samples from patients with previously untreated oropharyngeal carcinoma. Transcriptomes were analyzed using massively parallel messenger RNA sequencing and validated using the NanoString nCounter system. Global gene expression levels were compared in samples grouped by smoking status and human papillomavirus status. This study was completed between June 10, 2010, and June 30, 2011. Results: Global gene expression analysis indicated tumor tissue from former smokers grouped more closely to the never smokers than the current smokers. Pathway analysis revealed alterations in the expression of genes involved in the p53 DNA damage-repair pathway, including CHEK2 and ATR, which display patterns of increased expression that is associated with human papillomavirus-negative current smokers rather than former or never smokers. Conclusion: These findings support the application of messenger RNA sequencing technology as an important clinical tool for more accurately stratifying patients based on individual tumor biology with the goal of improving our understanding of tumor prognosis and treatment response, ultimately leading to individualized patient care strategies.
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UR - http://www.scopus.com/inward/citedby.url?scp=84863230211&partnerID=8YFLogxK
U2 - 10.1016/j.mayocp.2011.10.008
DO - 10.1016/j.mayocp.2011.10.008
M3 - Article
C2 - 22386177
AN - SCOPUS:84863230211
VL - 87
SP - 226
EP - 232
JO - Mayo Clinic Proceedings
JF - Mayo Clinic Proceedings
SN - 0025-6196
IS - 3
ER -