Transcriptional fingerprints of antigen-presenting cell subsets in the human vaginal mucosa and skin reflect tissue-specific immune microenvironments

Dorothée Duluc, Romain Banchereau, Julien Gannevat, Luann Thompson-Snipes, Jean Philippe Blanck, Sandra Zurawski, Gerard Zurawski, Seunghee Hong, Jose Rossello-Urgell, Virginia Pascual, Nicole Baldwin, Jack Stecher, Michael Carley, Muriel Boreham, SangKon Oh

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Background: Dendritic cells localize throughout the body, where they can sense and capture invading pathogens to induce protective immunity. Hence, harnessing the biology of tissue-resident dendritic cells is fundamental for the rational design of vaccines against pathogens. Methods: Herein, we characterized the transcriptomes of four antigen-presenting cell subsets from the human vagina (Langerhans cells, CD14- and CD14+ dendritic cells, macrophages) by microarray, at both the transcript and network level, and compared them to those of three skin dendritic cell subsets and blood myeloid dendritic cells. Results: We found that genomic fingerprints of antigen-presenting cells are significantly influenced by the tissue of origin as well as by individual subsets. Nonetheless, CD14+ populations from both vagina and skin are geared towards innate immunity and pro-inflammatory responses, whereas CD14- populations, particularly skin and vaginal Langerhans cells, and vaginal CD14- dendritic cells, display both Th2-inducing and regulatory phenotypes. We also identified new phenotypic and functional biomarkers of vaginal antigen-presenting cell subsets. Conclusions: We provide a transcriptional database of 87 microarray samples spanning eight antigen-presenting cell populations in the human vagina, skin and blood. Altogether, these data provide molecular information that will further help characterize human tissue antigen-presenting cell lineages and their functions. Data from this study can guide the design of mucosal vaccines against sexually transmitted pathogens.

Original languageEnglish (US)
Article number98
JournalGenome Medicine
Volume6
Issue number11
DOIs
StatePublished - Jan 1 2014
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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    Duluc, D., Banchereau, R., Gannevat, J., Thompson-Snipes, L., Blanck, J. P., Zurawski, S., Zurawski, G., Hong, S., Rossello-Urgell, J., Pascual, V., Baldwin, N., Stecher, J., Carley, M., Boreham, M., & Oh, S. (2014). Transcriptional fingerprints of antigen-presenting cell subsets in the human vaginal mucosa and skin reflect tissue-specific immune microenvironments. Genome Medicine, 6(11), [98]. https://doi.org/10.1186/s13073-014-0098-y