Transcriptional fingerprint of human whole blood at the site of coronary occlusion in acute myocardial infarction

Olivier Muller, Leen Delrue, Michalis Hamilos, Steven Vercauteren, Argyrios Ntalianis, Catalina Trana, Fabio Mangiacapra, Karen Dierickx, Bernard De Bruyne, William Wijns, Atta Behfar, Emanuele Barbato, Andre Terzic, Marc Vanderheyden, Jozef Bartunek

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Aims: Transcriptome patterns associated with acute myocardial infarction at the site of coronary occlusion are largely unknown. The aim of this study was to decipher the angiogenic, atherosclerotic, and inflammatory mRNA profiles in whole blood samples collected at the site of coronary occlusion in patients with ST-elevation myocardial infarction (STEMI). Methods and results: In five consecutive patients with STEMI, blood was sampled at the site of occlusion (local) and in the systemic circulation (peripheral) during primary percutaneous coronary intervention. RNA was extracted from whole blood samples. Among 221 genes involved in angiogenesis, inflammation and atherosclerosis, 24 were shown to be differentially modulated locally, by analysis with custom-designed DNA array technology. Validation in 28 distinct STEMI patients using real-time quantitative PCR identified seven out of these 24 genes to be consistently and significantly upregulated in local versus peripheral blood (p<0.05). Three genes were chemokine family members (CCL2, CCL18 and CXCL12), three genes belonged to the cell-cell and cell-extracellular matrix family (FN1, CDH5 and SPP1), and one gene was representative of the lipoprotein family (APOE). Conclusions: We identified a set of whole blood transcripts induced at the site of coronary occlusion in the acute phase of myocardial infarction. Resolved genes indicate a predominant role for chemokines, cell-extracellular matrix, and lipoprotein alterations in the pathophysiology of acute myocardial infarction and the initial response to myocardial injury.

Original languageEnglish (US)
Pages (from-to)458-466
Number of pages9
JournalEuroIntervention
Volume7
Issue number4
DOIs
StatePublished - Aug 1 2011

Keywords

  • Cytokines
  • Gene expression
  • Myocardial infarction
  • STEMI

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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    Muller, O., Delrue, L., Hamilos, M., Vercauteren, S., Ntalianis, A., Trana, C., Mangiacapra, F., Dierickx, K., De Bruyne, B., Wijns, W., Behfar, A., Barbato, E., Terzic, A., Vanderheyden, M., & Bartunek, J. (2011). Transcriptional fingerprint of human whole blood at the site of coronary occlusion in acute myocardial infarction. EuroIntervention, 7(4), 458-466. https://doi.org/10.4244/EIJV7I4A75