Transcription of the promoter of the rat NF-1 gene depends on the integrity of an Sp1 recognition site

Rosario Ammendola; Fotini Gounari; Giulia Piaggio; Vincenzo De Simone; Riccardo Cortese

doi:10.1128/MCB.10.1.387

Transcription of the promoter of the rat NF-1 gene depends on the integrity of an Sp1 recognition site

Rosario Ammendola, Fotini Gounari, Giulia Piaggio, Vincenzo De Simone, Riccardo Cortese

Immunology

Research output: Contribution to journal › Article › peer-review

Abstract

The transcription start site and promoter of the rat gene coding for the transcription factor NF-1 have been identified. The NF-1 promoter was fused to the chloramphenicol acetyltransferase-coding sequence, and the resulting plasmid was transcriptionally active in the HepG2 cell line. Footprinting and gel retardation analysis indicated that the transcription factor Sp1 binds to the NF-1 promoter. Mutants in the Sp1-binding site displayed a strong reduction in transcriptional activity.

Original language	English (US)
Pages (from-to)	387-390
Number of pages	4
Journal	Molecular and cellular biology
Volume	10
Issue number	1
DOIs	https://doi.org/10.1128/MCB.10.1.387
State	Published - Jan 1990

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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abstract = "The transcription start site and promoter of the rat gene coding for the transcription factor NF-1 have been identified. The NF-1 promoter was fused to the chloramphenicol acetyltransferase-coding sequence, and the resulting plasmid was transcriptionally active in the HepG2 cell line. Footprinting and gel retardation analysis indicated that the transcription factor Sp1 binds to the NF-1 promoter. Mutants in the Sp1-binding site displayed a strong reduction in transcriptional activity.",

author = "Rosario Ammendola and Fotini Gounari and Giulia Piaggio and {De Simone}, Vincenzo and Riccardo Cortese",

year = "1990",

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AU - Cortese, Riccardo

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Transcription of the promoter of the rat NF-1 gene depends on the integrity of an Sp1 recognition site

Abstract

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