Transcription of histone H4, H3, and H1 cell cycle genes: Promoter factor HiNF-D contains CDC2, cyclin A, and an RB-related protein

Andre J van Wijnen, Farah Aziz, Xavier Graña, Antonio De Luca, Rajesh K. Desai, Karen Jaarsveld, Thomas J. Last, Kenneth Soprano, Antonio Giordano, Jane B. Lian, Janet L. Stein, Gary S. Stein

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Cell cycle-controlled human histone genes are coordinately expressed during S phase, and transcriptional regulation involves a series of trans- acting factors (HiNFs). The proliferation-specific factor HiNF-D interacts with multiple recognition motifs in histone H4, H3, and H1 promoters. Using gel shift immunoassays, we show that CDC2, cyclin A, and an RB-related protein are ubiquitous subunits of HiNF-D binding activity isolated from several cell types. HiNF-D levels in vivo are sensitive to okadaic acid and staurosporine, indicating that HiNF-D activity and/or assembly is influenced by phosphorylation status. Thus, HiNF-D appears to be a multicomponent phosphoprotein that participates in coordinate control of multiple histone H4, H3, and H1 genes during the cell cycle. The presence of cell cycle mediators in the HiNF-D complex suggests linkage between transcriptional control of histones, enzymes involved in DNA synthesis, and the onset of DNA replication during the G1/S phase transition.

Original languageEnglish (US)
Pages (from-to)12882-12886
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number26
DOIs
StatePublished - Dec 20 1994
Externally publishedYes

Fingerprint

Cyclin A
cdc Genes
Histones
Proteins
S Phase
Cell Cycle
Okadaic Acid
Trans-Activators
Staurosporine
Phosphoproteins
Phase Transition
Protein Subunits
G1 Phase
DNA Replication
Immunoassay
Gels
Phosphorylation
DNA
Enzymes

Keywords

  • cell proliferation
  • cyclin-dependent kinase
  • gene expression
  • protein phosphorylation
  • tumor suppressor

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Transcription of histone H4, H3, and H1 cell cycle genes : Promoter factor HiNF-D contains CDC2, cyclin A, and an RB-related protein. / van Wijnen, Andre J; Aziz, Farah; Graña, Xavier; De Luca, Antonio; Desai, Rajesh K.; Jaarsveld, Karen; Last, Thomas J.; Soprano, Kenneth; Giordano, Antonio; Lian, Jane B.; Stein, Janet L.; Stein, Gary S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 26, 20.12.1994, p. 12882-12886.

Research output: Contribution to journalArticle

van Wijnen, AJ, Aziz, F, Graña, X, De Luca, A, Desai, RK, Jaarsveld, K, Last, TJ, Soprano, K, Giordano, A, Lian, JB, Stein, JL & Stein, GS 1994, 'Transcription of histone H4, H3, and H1 cell cycle genes: Promoter factor HiNF-D contains CDC2, cyclin A, and an RB-related protein', Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 26, pp. 12882-12886. https://doi.org/10.1073/pnas.91.26.12882
van Wijnen, Andre J ; Aziz, Farah ; Graña, Xavier ; De Luca, Antonio ; Desai, Rajesh K. ; Jaarsveld, Karen ; Last, Thomas J. ; Soprano, Kenneth ; Giordano, Antonio ; Lian, Jane B. ; Stein, Janet L. ; Stein, Gary S. / Transcription of histone H4, H3, and H1 cell cycle genes : Promoter factor HiNF-D contains CDC2, cyclin A, and an RB-related protein. In: Proceedings of the National Academy of Sciences of the United States of America. 1994 ; Vol. 91, No. 26. pp. 12882-12886.
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