Transcript identification in the BRCA1 candidate region

Barbara L. Weber, Kenneth J. Abel, Fergus J. Couch, Sofia Merajver, Lucio Castilla, Lawrence C. Brody, Francis S. Collins

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Chromosome 17q12-21 is known to contain a gene (or genes) which confers susceptibility to early-onset breast cancer and ovarian cancer (BRCA1). Identification and isolation of BRCA1 will likely provide the basis for increased understanding of the pathogenesis of breast and ovarian cancer, the development of targeted diagnostic and therapeutic approaches, and a means of screening women at risk of being BRCA1 mutation carriers. Genetic and physical maps of the BRCA1 candidate region have been largely completed and efforts are being directed at identification of candidate genes from within this region. We have begun the task of identifying transcripts from this region employing three complementary strategies. These include: 1) direct cDNA screening with cosmids derived from the BRCA1 region; 2) exon amplification; and 3) magnetic bead capture. Transcripts identified using these approaches are being characterized for: 1) tissue expression pattern; 2) the presence of genomic rearrangement in DNA derived from affected members of families believed to show linkage between breast cancer and genetic markers in the BRCA1 candidate interval; 3) altered size and/or expression pattern in RNA prepared from such individuals; and 4) homology to known genes or functional motifs. Germline mutations in affected individuals from these families will serve as presumptive evidence of BRCA1 identity.

Original languageEnglish (US)
Pages (from-to)115-124
Number of pages10
JournalBreast Cancer Research and Treatment
Volume33
Issue number2
DOIs
StatePublished - Feb 1 1995

Keywords

  • BRCA1
  • breast/ovarian cancer susceptibility gene
  • cDNA
  • exon amplification
  • molecular cloning
  • tumor suppressor gene

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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