@inbook{e9e0e196c2b7456fb71f1e9fabb4e53c,
title = "Tracking the transport of E-cadherin to and from the plasma membrane",
abstract = "The epithelial to mesenchymal transition (EMT) is the breakdown of epithelial cell morphology that gives way to a more mobile, mesenchymal phenotype. Although this process is fundamental to the development of multicellular organisms, it is also a key occurrence in many diseases, including cancers of epithelial origin E-cadherin is a central component of adherens junctions (AJs), which act as structural and signaling hubs in epithelial cells that oppose EMT. The loss of E-cadherin from the plasma membrane is an early indication of EMT and a marker of poor prognosis in many cancers making the trafficking of E-cadherin an area of great interest. Recent work from the authors' laboratory has established the role of type Iγ phosphatidylinositol 4-phosphate 5-kinase (PIPKIγ) in the trafficking of E-cadherin by studying the surface accessibility of E-cadherin in endocytosis and recycling assays. Additionally, immunofluorescence data demonstrated that cells lacking PIPKIγ lost E-cadherin at the plasma membrane. The biochemical and microscopic techniques used to investigate the trafficking of E-cadherin are presented herein.",
keywords = "E-cadherin, Endocytosis, PIPKIγ, Plasma membrane targeting, Recycling, Transport, Type Iγ phosphatidylinositol 4-phosphate 5-kinase trafficking",
author = "Wagoner, {Matthew P.} and Kun Ling and Anderson, {Richard A.}",
year = "2008",
doi = "10.1007/978-1-59745-261-8_20",
language = "English (US)",
isbn = "9781588299253",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "267--278",
booktitle = "Membrane Trafficking",
}