Tracking the transport of E-cadherin to and from the plasma membrane

Matthew P. Wagoner, Kun Ling, Richard A. Anderson

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The epithelial to mesenchymal transition (EMT) is the breakdown of epithelial cell morphology that gives way to a more mobile, mesenchymal phenotype. Although this process is fundamental to the development of multicellular organisms, it is also a key occurrence in many diseases, including cancers of epithelial origin E-cadherin is a central component of adherens junctions (AJs), which act as structural and signaling hubs in epithelial cells that oppose EMT. The loss of E-cadherin from the plasma membrane is an early indication of EMT and a marker of poor prognosis in many cancers making the trafficking of E-cadherin an area of great interest. Recent work from the authors' laboratory has established the role of type Iγ phosphatidylinositol 4-phosphate 5-kinase (PIPKIγ) in the trafficking of E-cadherin by studying the surface accessibility of E-cadherin in endocytosis and recycling assays. Additionally, immunofluorescence data demonstrated that cells lacking PIPKIγ lost E-cadherin at the plasma membrane. The biochemical and microscopic techniques used to investigate the trafficking of E-cadherin are presented herein.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages267-278
Number of pages12
Volume457
DOIs
StatePublished - 2008
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume457
ISSN (Print)10643745

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Keywords

  • E-cadherin
  • Endocytosis
  • PIPKIγ
  • Plasma membrane targeting
  • Recycling
  • Transport
  • Type Iγ phosphatidylinositol 4-phosphate 5-kinase trafficking

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Wagoner, M. P., Ling, K., & Anderson, R. A. (2008). Tracking the transport of E-cadherin to and from the plasma membrane. In Methods in Molecular Biology (Vol. 457, pp. 267-278). (Methods in Molecular Biology; Vol. 457). https://doi.org/10.1007/978-1-59745-261-8-20