TP53 gene mutations and 17p deletions in human astrocytomas

R. Chung, J. Whaley, N. Kley, K. Anderson, D. Louis, A. Menon, C. Hettlich, R. Freiman, E. T. Hedley-Whyte, R. Martuza, Robert Brian Jenkins, D. Yandell, B. R. Seizinger

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Abstract

Astrocytomas, including the most malignant form, glioblastoma multiforme, are the most frequent and deadly primary tumors of the human nervous system. Recent molecular genetic analyses of astrocytomas have demonstrated frequent chromosome 17 deletions involving the telomeric region of the short arm (17p12-pter). This region contains a candidate tumor suppressor gene, TP53, which has recently been implicated in the etiology of a broad array of human cancers. To study the possible role of TP53 in astrocytoma development, 24 randomly chosen human astrocytic tumors were examined for genomic TP53 sequence aberrations using primer-directed DNA amplification in conjunction with direct sequencing. Five of the 11 grade 111 astrocytomas (glioblastoma multiforme), but only one of seven grade 11 astrocytomas (anaplastic astrocytoma) and none of either the grade 1 astrocytomas or oligodendrogliomas demonstrated distinct point mutations involving the TP53 gene. These data suggest that TP53 mutations may play a role in astrocytoma development and are predominantly associated with higher grade tumors.

Original languageEnglish (US)
Pages (from-to)323-331
Number of pages9
JournalGenes Chromosomes and Cancer
Volume3
Issue number5
DOIs
StatePublished - 1991

Fingerprint

Sequence Deletion
p53 Genes
Astrocytoma
Glioblastoma
Nervous System Neoplasms
Oligodendroglioma
Neoplasms
DNA Primers
Tumor Suppressor Genes
Point Mutation
Molecular Biology
Mutation

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

Cite this

Chung, R., Whaley, J., Kley, N., Anderson, K., Louis, D., Menon, A., ... Seizinger, B. R. (1991). TP53 gene mutations and 17p deletions in human astrocytomas. Genes Chromosomes and Cancer, 3(5), 323-331. https://doi.org/10.1002/gcc.2870030502

TP53 gene mutations and 17p deletions in human astrocytomas. / Chung, R.; Whaley, J.; Kley, N.; Anderson, K.; Louis, D.; Menon, A.; Hettlich, C.; Freiman, R.; Hedley-Whyte, E. T.; Martuza, R.; Jenkins, Robert Brian; Yandell, D.; Seizinger, B. R.

In: Genes Chromosomes and Cancer, Vol. 3, No. 5, 1991, p. 323-331.

Research output: Contribution to journalArticle

Chung, R, Whaley, J, Kley, N, Anderson, K, Louis, D, Menon, A, Hettlich, C, Freiman, R, Hedley-Whyte, ET, Martuza, R, Jenkins, RB, Yandell, D & Seizinger, BR 1991, 'TP53 gene mutations and 17p deletions in human astrocytomas', Genes Chromosomes and Cancer, vol. 3, no. 5, pp. 323-331. https://doi.org/10.1002/gcc.2870030502
Chung R, Whaley J, Kley N, Anderson K, Louis D, Menon A et al. TP53 gene mutations and 17p deletions in human astrocytomas. Genes Chromosomes and Cancer. 1991;3(5):323-331. https://doi.org/10.1002/gcc.2870030502
Chung, R. ; Whaley, J. ; Kley, N. ; Anderson, K. ; Louis, D. ; Menon, A. ; Hettlich, C. ; Freiman, R. ; Hedley-Whyte, E. T. ; Martuza, R. ; Jenkins, Robert Brian ; Yandell, D. ; Seizinger, B. R. / TP53 gene mutations and 17p deletions in human astrocytomas. In: Genes Chromosomes and Cancer. 1991 ; Vol. 3, No. 5. pp. 323-331.
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