TY - JOUR
T1 - Toxic reactions evoked by glutaraldehyde‐fixed pericardium and cardiac valve tissue bioprosthesis
AU - Gendler, E.
AU - Gendler, S.
AU - Nimni, M. E.
PY - 1984/9
Y1 - 1984/9
N2 - Bovine pericardial tissue and tissue‐derived bioprosthesis fixed in glutaraldehyde and stored in either glutaraldehyde or formaldehyde can induce cytotoxic reactions even after prolonged washing due to the slow leaching of the chemicals used for crosslinking and sterilizing. Sequential rinsing for up to 60 min was still not sufficient to eliminate cytotoxic effects. On the other hand, satisfactory results were obtained when cardiac valves made from glutaraldehyde fixed pericardium were stored in a solution containing 0.02% of propyl‐hydroxy‐benzoate and 0.18% methyl‐hydroxy‐benzoate. The valves stored in this solution and rinsed three times for 1 min in normal saline showed few signs of residual cytotoxicity. Rinsing in a solution containing glycine was partially effective in neutralizing the cytotoxic effect, and this or similar approaches offer good possibilities. The use of Chang cells grown in culture provides an excellent quantitative assay for the assessment of residual cytotoxicity and for evaluating the host response to different methods of fixation, storage, and rinsing of tissue derived bioprosthesis.
AB - Bovine pericardial tissue and tissue‐derived bioprosthesis fixed in glutaraldehyde and stored in either glutaraldehyde or formaldehyde can induce cytotoxic reactions even after prolonged washing due to the slow leaching of the chemicals used for crosslinking and sterilizing. Sequential rinsing for up to 60 min was still not sufficient to eliminate cytotoxic effects. On the other hand, satisfactory results were obtained when cardiac valves made from glutaraldehyde fixed pericardium were stored in a solution containing 0.02% of propyl‐hydroxy‐benzoate and 0.18% methyl‐hydroxy‐benzoate. The valves stored in this solution and rinsed three times for 1 min in normal saline showed few signs of residual cytotoxicity. Rinsing in a solution containing glycine was partially effective in neutralizing the cytotoxic effect, and this or similar approaches offer good possibilities. The use of Chang cells grown in culture provides an excellent quantitative assay for the assessment of residual cytotoxicity and for evaluating the host response to different methods of fixation, storage, and rinsing of tissue derived bioprosthesis.
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U2 - 10.1002/jbm.820180703
DO - 10.1002/jbm.820180703
M3 - Article
C2 - 6085799
AN - SCOPUS:0021489009
SN - 0021-9304
VL - 18
SP - 727
EP - 736
JO - Journal of Biomedical Materials Research
JF - Journal of Biomedical Materials Research
IS - 7
ER -