Towards improved biomarker studies of cervical neoplasia

Sophia S. Wang, Abhijit Dasgupta, Mark E. Sherman, Joan L. Walker, Michael A. Gold, Rosemary Zuna, Lori Sakoda, Sholom Wacholder, Mark Schiffman, Carl C. Baker

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Among tumor sites, cervical cancer offers an ideal model for investigating differences in gene expression associated with transitions from normal to precancer and invasion to cancer. To evaluate the validity of assessing gene expression in cervical tissues acquired in a clinical setting, we investigated whether standard procedures, namely the application of acetic acid and/or Lugol's iodine, employed for the visualization of colposcopically directed biopsies, altered patterns in oligonucleotide (oligo) arrays. We compared microarray profiles from six women, each with three adjacent tissue samples removed from benign hysterectomy specimens and treated as follows: immediately frozen, acetic acid application only, acetic acid, and Lugol's iodine. Of the 22,464 original spots on the microarray, 4,850 spots were expressed at detectable levels for further evaluation upon data normalization and filtration. For each spot, the difference between topical applications was computed, and P values were calculated using a bivariate T 2 test. Upon adjustment for multiple comparisons using both the Holm's and Hochberg's procedures as well as the False Discovery Rate (Benjamini-Hochberg and Benjamini-Yeuketili [BY]), we failed to identify genes differentially expressed and conclude that standard precolposcopic procedures do not substantially affect the overall gene expression patterns in the normal cervix.

Original languageEnglish (US)
Pages (from-to)59-64
Number of pages6
JournalDiagnostic Molecular Pathology
Volume14
Issue number2
DOIs
StatePublished - Jun 1 2005

Keywords

  • Gene expression
  • Microarray
  • Tissue acquisition

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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    Wang, S. S., Dasgupta, A., Sherman, M. E., Walker, J. L., Gold, M. A., Zuna, R., Sakoda, L., Wacholder, S., Schiffman, M., & Baker, C. C. (2005). Towards improved biomarker studies of cervical neoplasia. Diagnostic Molecular Pathology, 14(2), 59-64. https://doi.org/10.1097/01.pas.0000162754.45908.d4