Topoisomerase IIα controls the decatenation checkpoint

Kuntian Luo, Jian Yuan, Junjie Chen, Zhenkun Lou

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Topoisomerase II (Topo II) is required to separate intertwined sister chromatids before chromosome segregation can occur in mitosis. However, it remains to be resolved whether Topo II has any role in checkpoint control. Here we report that when phosphorylated, Ser 1524 of Topo IIα acts as a binding site for the BRCT domain of MDC1 (mediator of DNA damage checkpoint protein-1), thereby recruiting MDC1 to chromatin. Although Topo IIα-MDC1 interaction is not required for checkpoint activation induced by DNA damage, it is required for activation of the decatenation checkpoint. Mutation of Ser 1524 results in a defective decatenation checkpoint. These results reveal an important role of Topo II in checkpoint activation and in the maintenance of genomic stability.

Original languageEnglish (US)
Pages (from-to)204-210
Number of pages7
JournalNature Cell Biology
Volume11
Issue number2
DOIs
StatePublished - 2009

Fingerprint

Type II DNA Topoisomerase
DNA Damage
Chromosome Segregation
Chromatids
Genomic Instability
Mitosis
Chromatin
Binding Sites
Maintenance
Mutation
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Topoisomerase IIα controls the decatenation checkpoint. / Luo, Kuntian; Yuan, Jian; Chen, Junjie; Lou, Zhenkun.

In: Nature Cell Biology, Vol. 11, No. 2, 2009, p. 204-210.

Research output: Contribution to journalArticle

Luo, Kuntian ; Yuan, Jian ; Chen, Junjie ; Lou, Zhenkun. / Topoisomerase IIα controls the decatenation checkpoint. In: Nature Cell Biology. 2009 ; Vol. 11, No. 2. pp. 204-210.
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