Toll-Like Receptors Induce Signal-Specific Reprogramming of the Macrophage Lipidome

Wei Yuan Hsieh, Quan D. Zhou, Autumn G. York, Kevin J. Williams, Philip O. Scumpia, Eliza B. Kronenberger, Xen Ping Hoi, Baolong Su, Xun Chi, Viet L. Bui, Elvira Khialeeva, Amber Kaplan, Young Min Son, Ajit S. Divakaruni, Jie Sun, Stephen T. Smale, Richard A. Flavell, Steven J. Bensinger

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Macrophages reprogram their lipid metabolism in response to activation signals. However, a systems-level understanding of how different pro-inflammatory stimuli reshape the macrophage lipidome is lacking. Here, we use complementary “shotgun” and isotope tracer mass spectrometry approaches to define the changes in lipid biosynthesis, import, and composition of macrophages induced by various Toll-like receptors (TLRs) and inflammatory cytokines. “Shotgun” lipidomics data revealed that different TLRs and cytokines induce macrophages to acquire distinct lipidomes, indicating their specificity in reshaping lipid composition. Mechanistic studies showed that differential reprogramming of lipid composition is mediated by the opposing effects of MyD88- and TRIF-interferon-signaling pathways. Finally, we applied these insights to show that perturbing reprogramming of lipid composition can enhance inflammation and promote host defense to bacterial challenge. These studies provide a framework for understanding how inflammatory stimuli reprogram lipid composition of macrophages while providing a knowledge platform to exploit differential lipidomics to influence immunity.

Original languageEnglish (US)
Pages (from-to)128-143.e5
JournalCell Metabolism
Issue number1
StatePublished - Jul 7 2020


  • MyD88
  • acetylated-LDL
  • host defense
  • inflammation
  • interferon
  • lipidomics
  • macrophages
  • stable isotope tracer analysis
  • stearoyl-CoA desaturase
  • toll-like receptors

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology


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