TNF-α system response in a rat model of very preterm brain injuries induced by lipopolysaccharide and/or hypoxia-ischemia

Mélanie Roy, Sylvie Girard, Annie Larouche, Hazim Kadhim, Guillaume Sébire

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: The aim of this study was to determine, with the use of a rat model, the expression of tumor necrosis factor (TNF)-α, its receptors, and TNF-α-converting enzyme in perinatal brain lesions of early premature neonates. Study Design: Lipopolysaccharide (LPS) was injected intraperitoneally in pregnant rats at the end of gestation. At postnatal day 1, the right carotid artery was ligated and followed by exposure to hypoxia. Forebrains (n = 220) were collected to study the TNF-α system. Results: LPS alone or combined with hypoxia-ischemia (HI) led to a slight decrease of intracerebral TNF-α, whereas sole HI induced no variation. TNF-α-converting enzyme followed the same pattern of expression as TNF-α. TNF receptor 1 was up-regulated in forebrains that were submitted to LPS alone or combined with HI. No variation was observed in TNF receptor 2 expression. Conclusion: The minimal expression of the TNF-α system that we observed may indicate that this pathway is not central in the pathogenesis of brain lesions in early premature neonates.

Original languageEnglish (US)
Pages (from-to)493.e1-493.e10
JournalAmerican journal of obstetrics and gynecology
Volume201
Issue number5
DOIs
StatePublished - Nov 2009

Keywords

  • TNF-α
  • cerebral palsy
  • neonatal brain lesions
  • rat
  • very preterm neonate

ASJC Scopus subject areas

  • Obstetrics and Gynecology

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