TL1A synergizes with IL-12 and IL-18 to enhance IFN-γ production in human T cells and NK cells

Konstantinos Papadakis, John L. Prehn, Carol Landers, Qiwei Han, Xia Luo, Stephanie C. Cha, Ping Wei, Stephan R. Targan

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

TL1A, a recently described TNF-like cytokine that interacts with DR3, costimulates T cells and augments anti-CD3 plus anti-CD28 IFN-γ production. In the current study we show that TL1A or an agonistic anti-DR3 mAb synergize with IL-12/IL-18 to augment IFN-γ production in human peripheral blood T cells and NK cells. TL1A also enhanced IFN-γ production by IL-12/IL-18 stimulated CD56+ T cells. When expressed as fold change, the synergistic effect of TL1A on cytokine-induced IFN-γ production was more pronounced on CD4+ and CD8+ T cells than on CD56+ T cells or NK cells. Intracellular cytokine staining showed that TL1A significantly enhanced both the percentage and the mean fluorescence intensity of IFN-γ-producing T cells in response to IL-12/IL-48. The combination of IL-12 and IL-18 markedly up-regulated DR3 expression in NK cells, whereas it had minimal effect in T cells. Our data suggest that TL1A/DR3 pathway plays an important role in the augmentation of cytokine-induced IFN-γ production in T cells and that DR3 expression is differentially regulated by IL-17/IL-18 in T cells and NK cells.

Original languageEnglish (US)
Pages (from-to)7002-7007
Number of pages6
JournalJournal of Immunology
Volume172
Issue number11
StatePublished - Jun 1 2004
Externally publishedYes

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Interleukin-18
Interleukin-12
Natural Killer Cells
T-Lymphocytes
Cytokines
Interleukin-17
Blood Cells
Fluorescence
Staining and Labeling

ASJC Scopus subject areas

  • Immunology

Cite this

Papadakis, K., Prehn, J. L., Landers, C., Han, Q., Luo, X., Cha, S. C., ... Targan, S. R. (2004). TL1A synergizes with IL-12 and IL-18 to enhance IFN-γ production in human T cells and NK cells. Journal of Immunology, 172(11), 7002-7007.

TL1A synergizes with IL-12 and IL-18 to enhance IFN-γ production in human T cells and NK cells. / Papadakis, Konstantinos; Prehn, John L.; Landers, Carol; Han, Qiwei; Luo, Xia; Cha, Stephanie C.; Wei, Ping; Targan, Stephan R.

In: Journal of Immunology, Vol. 172, No. 11, 01.06.2004, p. 7002-7007.

Research output: Contribution to journalArticle

Papadakis, K, Prehn, JL, Landers, C, Han, Q, Luo, X, Cha, SC, Wei, P & Targan, SR 2004, 'TL1A synergizes with IL-12 and IL-18 to enhance IFN-γ production in human T cells and NK cells', Journal of Immunology, vol. 172, no. 11, pp. 7002-7007.
Papadakis, Konstantinos ; Prehn, John L. ; Landers, Carol ; Han, Qiwei ; Luo, Xia ; Cha, Stephanie C. ; Wei, Ping ; Targan, Stephan R. / TL1A synergizes with IL-12 and IL-18 to enhance IFN-γ production in human T cells and NK cells. In: Journal of Immunology. 2004 ; Vol. 172, No. 11. pp. 7002-7007.
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