Titration of feline immunodeficiency virus-based lentiviral vector preparations

Dyana T. Saenz, Román Barraza, Nils Loewen, Wulin Teo, Eric M. Poeschla

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Feline immunodeficiency virus (FIV)-based lentiviral vectors are useful for introducing integrated transgenes into nondividing human cells. This protocol describes methods for measuring and calculating vector titers in transducing units (TU)/mL. Alternate methods are provided for green fluorescent protein (GFP) vectors and for β-galactosidase vectors.

Original languageEnglish (US)
Pages (from-to)126-128
Number of pages3
JournalCold Spring Harbor Protocols
Volume7
Issue number1
DOIs
StatePublished - Jan 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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