Tissue specific binding in vitro of progesterone-receptor to the chromatins of chick tissues

A. W. Steggles, T. C. Spelsberg, B. W. O'Malley

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

When H3-progesterone is incubated with chick oviduct cytosol, it binds to a specific receptor protein. The subsequent binding of this complex to oviduct chromatin is greater than that of free progesterone alone. This specific affinity for the progesterone-receptor complex is not observed with spleen, heart, liver or erythrocyte chromatin. Progesterone incubated with the cytosols of non-target tissues such as liver or spleen shows no specific binding to chromatin from any source. Artificial complexes of chick histone and DNA display a low degree of non-specific binding. The results suggest that the chromatin of target tissue (oviduct) may contain "acceptor" sites for the hormone-receptor complex contained in the cytoplasm of the cell. The genome may thus be preprogrammed to receive the hormone receptor complex as it enters the nucleus of the target cell.

Original languageEnglish (US)
Pages (from-to)20-27
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume43
Issue number1
DOIs
StatePublished - Apr 2 1971
Externally publishedYes

Fingerprint

Progesterone Receptors
Chromatin
Oviducts
Tissue
Progesterone
Liver
Cytosol
Spleen
Hormones
Cell Nucleus
Histones
Cytoplasm
Genes
Erythrocytes
Genome
In Vitro Techniques
DNA
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Tissue specific binding in vitro of progesterone-receptor to the chromatins of chick tissues. / Steggles, A. W.; Spelsberg, T. C.; O'Malley, B. W.

In: Biochemical and Biophysical Research Communications, Vol. 43, No. 1, 02.04.1971, p. 20-27.

Research output: Contribution to journalArticle

Steggles, A. W. ; Spelsberg, T. C. ; O'Malley, B. W. / Tissue specific binding in vitro of progesterone-receptor to the chromatins of chick tissues. In: Biochemical and Biophysical Research Communications. 1971 ; Vol. 43, No. 1. pp. 20-27.
@article{82206ca622c64fd48343fa2a5dc94517,
title = "Tissue specific binding in vitro of progesterone-receptor to the chromatins of chick tissues",
abstract = "When H3-progesterone is incubated with chick oviduct cytosol, it binds to a specific receptor protein. The subsequent binding of this complex to oviduct chromatin is greater than that of free progesterone alone. This specific affinity for the progesterone-receptor complex is not observed with spleen, heart, liver or erythrocyte chromatin. Progesterone incubated with the cytosols of non-target tissues such as liver or spleen shows no specific binding to chromatin from any source. Artificial complexes of chick histone and DNA display a low degree of non-specific binding. The results suggest that the chromatin of target tissue (oviduct) may contain {"}acceptor{"} sites for the hormone-receptor complex contained in the cytoplasm of the cell. The genome may thus be preprogrammed to receive the hormone receptor complex as it enters the nucleus of the target cell.",
author = "Steggles, {A. W.} and Spelsberg, {T. C.} and O'Malley, {B. W.}",
year = "1971",
month = "4",
day = "2",
doi = "10.1016/S0006-291X(71)80079-7",
language = "English (US)",
volume = "43",
pages = "20--27",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Tissue specific binding in vitro of progesterone-receptor to the chromatins of chick tissues

AU - Steggles, A. W.

AU - Spelsberg, T. C.

AU - O'Malley, B. W.

PY - 1971/4/2

Y1 - 1971/4/2

N2 - When H3-progesterone is incubated with chick oviduct cytosol, it binds to a specific receptor protein. The subsequent binding of this complex to oviduct chromatin is greater than that of free progesterone alone. This specific affinity for the progesterone-receptor complex is not observed with spleen, heart, liver or erythrocyte chromatin. Progesterone incubated with the cytosols of non-target tissues such as liver or spleen shows no specific binding to chromatin from any source. Artificial complexes of chick histone and DNA display a low degree of non-specific binding. The results suggest that the chromatin of target tissue (oviduct) may contain "acceptor" sites for the hormone-receptor complex contained in the cytoplasm of the cell. The genome may thus be preprogrammed to receive the hormone receptor complex as it enters the nucleus of the target cell.

AB - When H3-progesterone is incubated with chick oviduct cytosol, it binds to a specific receptor protein. The subsequent binding of this complex to oviduct chromatin is greater than that of free progesterone alone. This specific affinity for the progesterone-receptor complex is not observed with spleen, heart, liver or erythrocyte chromatin. Progesterone incubated with the cytosols of non-target tissues such as liver or spleen shows no specific binding to chromatin from any source. Artificial complexes of chick histone and DNA display a low degree of non-specific binding. The results suggest that the chromatin of target tissue (oviduct) may contain "acceptor" sites for the hormone-receptor complex contained in the cytoplasm of the cell. The genome may thus be preprogrammed to receive the hormone receptor complex as it enters the nucleus of the target cell.

UR - http://www.scopus.com/inward/record.url?scp=0015207861&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015207861&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(71)80079-7

DO - 10.1016/S0006-291X(71)80079-7

M3 - Article

C2 - 5579944

AN - SCOPUS:0015207861

VL - 43

SP - 20

EP - 27

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -