Tissue specific binding in vitro of progesterone-receptor to the chromatins of chick tissues

A. W. Steggles, T. C. Spelsberg, B. W. O'Malley

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

When H3-progesterone is incubated with chick oviduct cytosol, it binds to a specific receptor protein. The subsequent binding of this complex to oviduct chromatin is greater than that of free progesterone alone. This specific affinity for the progesterone-receptor complex is not observed with spleen, heart, liver or erythrocyte chromatin. Progesterone incubated with the cytosols of non-target tissues such as liver or spleen shows no specific binding to chromatin from any source. Artificial complexes of chick histone and DNA display a low degree of non-specific binding. The results suggest that the chromatin of target tissue (oviduct) may contain "acceptor" sites for the hormone-receptor complex contained in the cytoplasm of the cell. The genome may thus be preprogrammed to receive the hormone receptor complex as it enters the nucleus of the target cell.

Original languageEnglish (US)
Pages (from-to)20-27
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume43
Issue number1
DOIs
StatePublished - Apr 2 1971

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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