TY - JOUR
T1 - Tissue factor pathway inhibitor induces expression of JUNB and GADD45B mRNAs
AU - Shirotani-Ikejima, Hiroko
AU - Kokame, Koichi
AU - Hamuro, Tsutomu
AU - Bu, Guojun
AU - Kato, Hisao
AU - Miyata, Toshiyuki
N1 - Funding Information:
We thank Dr. Takehiko Koide (The Himeji Institute of Technology) for his kind donation of antithrombin and Dr. Jane Kinsely (Washington University School of Medicine) for critical reading of the manuscript. This work was supported in part by Grants-in-Aid from the Ministry of Health, Labour and Welfare of Japan; from the Ministry of Education, Culture, Sports, Science and Technology of Japan; from the Japan Society for the Promotion of Science; and by the Program for Promotion of Fundamental Studies in Health Sciences of the Organization for Pharmaceutical Safety and Research of Japan.
PY - 2002
Y1 - 2002
N2 - Tissue factor pathway inhibitor (TFPI) is a Kunitz-type protease inhibitor that regulates tissue factor-triggered blood coagulation. It has previously been reported that TFPI inhibits the proliferation of human umbilical vein endothelial cells (HUVECs), suggesting that TFPI may act as more than just a mediator of coagulation through changes in gene expression. By using DNA-array techniques and Northern blot analysis, we here revealed that TFPI transiently induced the mRNA expression of JUNB and GADD45B. The inducible effects were not observed in TFPIDC (lacking the C-terminal basic region) or antithrombin (heparin-binding anticoagulant protease inhibitor). Moreover, the TFPI-induced expression of GADD45B was blocked by receptor-associated protein, which masks the ligand-binding domain of very low density lipoprotein receptor (VLDL-R). In conclusion, this is the first report to show an effect of TFPI on mRNA expression, and suggests that TFPI modulates cellular functions by inducing JUNB and GADD45B expression through binding to VLDL-R.
AB - Tissue factor pathway inhibitor (TFPI) is a Kunitz-type protease inhibitor that regulates tissue factor-triggered blood coagulation. It has previously been reported that TFPI inhibits the proliferation of human umbilical vein endothelial cells (HUVECs), suggesting that TFPI may act as more than just a mediator of coagulation through changes in gene expression. By using DNA-array techniques and Northern blot analysis, we here revealed that TFPI transiently induced the mRNA expression of JUNB and GADD45B. The inducible effects were not observed in TFPIDC (lacking the C-terminal basic region) or antithrombin (heparin-binding anticoagulant protease inhibitor). Moreover, the TFPI-induced expression of GADD45B was blocked by receptor-associated protein, which masks the ligand-binding domain of very low density lipoprotein receptor (VLDL-R). In conclusion, this is the first report to show an effect of TFPI on mRNA expression, and suggests that TFPI modulates cellular functions by inducing JUNB and GADD45B expression through binding to VLDL-R.
KW - GADD45B
KW - JUNB
KW - Tissue factor pathway inhibitor
KW - Very low density lipoprotein receptor
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U2 - 10.1016/S0006-291X(02)02759-6
DO - 10.1016/S0006-291X(02)02759-6
M3 - Article
C2 - 12470656
AN - SCOPUS:0036929420
SN - 0006-291X
VL - 299
SP - 847
EP - 852
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 5
ER -