Tissue factor pathway inhibitor induces expression of JUNB and GADD45B mRNAs

Hiroko Shirotani-Ikejima, Koichi Kokame, Tsutomu Hamuro, Guojun Bu, Hisao Kato, Toshiyuki Miyata

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Tissue factor pathway inhibitor (TFPI) is a Kunitz-type protease inhibitor that regulates tissue factor-triggered blood coagulation. It has previously been reported that TFPI inhibits the proliferation of human umbilical vein endothelial cells (HUVECs), suggesting that TFPI may act as more than just a mediator of coagulation through changes in gene expression. By using DNA-array techniques and Northern blot analysis, we here revealed that TFPI transiently induced the mRNA expression of JUNB and GADD45B. The inducible effects were not observed in TFPIDC (lacking the C-terminal basic region) or antithrombin (heparin-binding anticoagulant protease inhibitor). Moreover, the TFPI-induced expression of GADD45B was blocked by receptor-associated protein, which masks the ligand-binding domain of very low density lipoprotein receptor (VLDL-R). In conclusion, this is the first report to show an effect of TFPI on mRNA expression, and suggests that TFPI modulates cellular functions by inducing JUNB and GADD45B expression through binding to VLDL-R.

Original languageEnglish (US)
Pages (from-to)847-852
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume299
Issue number5
DOIs
StatePublished - Dec 1 2002

Keywords

  • GADD45B
  • JUNB
  • Tissue factor pathway inhibitor
  • Very low density lipoprotein receptor

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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