TY - JOUR
T1 - Time-resolved fluorescence polarization from ordered biological assemblies
AU - Burghardt, T. P.
PY - 1985
Y1 - 1985
N2 - We calculate the time dependence of the polarized fluorescence signal from fluorescent-labeled elements of a biological assembly that are rotationally diffusing in an arbitrary three-dimensional angular potential. We have formulated this calculation using the model-independent description of the angular potential wherein the angular potential is described by an expansion in a complete set of orthonormal functions with the expansion coefficients (or order parameters) determined by time-independent methods (Burghardt, T. P., 1984, Biopolymers, 23:2382–2406). We have applied the calculation to fluorescent-labeled myosin cross-bridges in relaxed muscle fibers. In a related paper we describe the experimental observation of the myosin cross-bridges rotationally diffusing in an angular potential (Burghardt, T. P., and N. L. Thompson, 1985, Biochemistry, 24:3731–3735).
AB - We calculate the time dependence of the polarized fluorescence signal from fluorescent-labeled elements of a biological assembly that are rotationally diffusing in an arbitrary three-dimensional angular potential. We have formulated this calculation using the model-independent description of the angular potential wherein the angular potential is described by an expansion in a complete set of orthonormal functions with the expansion coefficients (or order parameters) determined by time-independent methods (Burghardt, T. P., 1984, Biopolymers, 23:2382–2406). We have applied the calculation to fluorescent-labeled myosin cross-bridges in relaxed muscle fibers. In a related paper we describe the experimental observation of the myosin cross-bridges rotationally diffusing in an angular potential (Burghardt, T. P., and N. L. Thompson, 1985, Biochemistry, 24:3731–3735).
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U2 - 10.1016/S0006-3495(85)83818-2
DO - 10.1016/S0006-3495(85)83818-2
M3 - Article
C2 - 4052569
AN - SCOPUS:0022401754
SN - 0006-3495
VL - 48
SP - 623
EP - 631
JO - Biophysical Journal
JF - Biophysical Journal
IS - 4
ER -