Time-resolved fluorescence polarization from ordered biological assemblies

Thomas P Burghardt

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We calculate the time dependence of the polarized fluorescence signal from fluorescent-labeled elements of a biological assembly that are rotationally diffusing in an arbitrary three-dimensional angular potential. We have formulated this calculation using the model-independent description of the angular potential where in the angular potential is described by an expansion in a complete set of orthonormal functions with the expansion coefficients (or order parameters) determined by time-independent methods (Burghardt, T.P., 1984, Biopolymers, 23:2382-2406). We have applied the calculation to fluorescent-labeled myosin cross-bridges in relaxed muscle fibers. In a related paper we describe the experimental observation of the myosin cross-bridge rotationally diffusing in an angular potential (Burghardt, T.P., and N.L. Thompson, 1985, Biochemistry, 24:3731-3735).

Original languageEnglish (US)
Pages (from-to)623-631
Number of pages9
JournalBiophysical Journal
Volume48
Issue number4
StatePublished - 1985
Externally publishedYes

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Fluorescence Polarization
Myosins
Biopolymers
Biochemistry
Fluorescence
Observation
Muscles

ASJC Scopus subject areas

  • Biophysics

Cite this

Time-resolved fluorescence polarization from ordered biological assemblies. / Burghardt, Thomas P.

In: Biophysical Journal, Vol. 48, No. 4, 1985, p. 623-631.

Research output: Contribution to journalArticle

Burghardt, Thomas P. / Time-resolved fluorescence polarization from ordered biological assemblies. In: Biophysical Journal. 1985 ; Vol. 48, No. 4. pp. 623-631.
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