Time dependence of the effect of ethanol on serotonin synthesis and tryptophan hydroxylase expression in the dorsal and median raphe of young rats

Mi Hyeon Jang; Min Chul Shin; Hong Kim; Ee Hwa Kim; Chang Ju Kim

doi:10.1002/nrc.10085

Time dependence of the effect of ethanol on serotonin synthesis and tryptophan hydroxylase expression in the dorsal and median raphe of young rats

Mi Hyeon Jang, Min Chul Shin, Hong Kim, Ee Hwa Kim, Chang Ju Kim

Neurosurgery

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Abstract

Serotonin (5-hydroxytryptamine, 5-HT) has been implicated in the pathophysiology of many neuropsychiatric disorders, and alcohol is reported to influence serotonergic neuronal activity in the dorsal raphe. In the present study, the effects of ethanol on the synthesis of 5-HT and the expression of tryptophan hydroxylase (TPH), the rate-limiting enzyme of 5-HT synthesis, in the dorsal and median raphe of rats were investigated, using immunohistochemical methods. Results showed that the density of 5-HT-positive and TPH-positive cells was reduced by ethanol treatment in a time-dependent manner. Based on these results, it is suggested that the pathogenesis of ethanol-induced neuropsychological disorders involves ethanol-induced suppression of 5-HT synthesis and TPH expression in the raphe region.

Original language	English (US)
Pages (from-to)	99-104
Number of pages	6
Journal	Neuroscience Research Communications
Volume	33
Issue number	2
DOIs	https://doi.org/10.1002/nrc.10085
State	Published - Sep 2003

Keywords

Adolescence
Ethanol
Immunohistochemistry
Serotonin
Tryptophan hydroxylase

ASJC Scopus subject areas

General Neuroscience

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10.1002/nrc.10085

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title = "Time dependence of the effect of ethanol on serotonin synthesis and tryptophan hydroxylase expression in the dorsal and median raphe of young rats",

abstract = "Serotonin (5-hydroxytryptamine, 5-HT) has been implicated in the pathophysiology of many neuropsychiatric disorders, and alcohol is reported to influence serotonergic neuronal activity in the dorsal raphe. In the present study, the effects of ethanol on the synthesis of 5-HT and the expression of tryptophan hydroxylase (TPH), the rate-limiting enzyme of 5-HT synthesis, in the dorsal and median raphe of rats were investigated, using immunohistochemical methods. Results showed that the density of 5-HT-positive and TPH-positive cells was reduced by ethanol treatment in a time-dependent manner. Based on these results, it is suggested that the pathogenesis of ethanol-induced neuropsychological disorders involves ethanol-induced suppression of 5-HT synthesis and TPH expression in the raphe region.",

keywords = "Adolescence, Ethanol, Immunohistochemistry, Serotonin, Tryptophan hydroxylase",

author = "Jang, {Mi Hyeon} and Shin, {Min Chul} and Hong Kim and Kim, {Ee Hwa} and Kim, {Chang Ju}",

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AU - Jang, Mi Hyeon

AU - Shin, Min Chul

AU - Kim, Hong

AU - Kim, Ee Hwa

AU - Kim, Chang Ju

PY - 2003/9

Y1 - 2003/9

N2 - Serotonin (5-hydroxytryptamine, 5-HT) has been implicated in the pathophysiology of many neuropsychiatric disorders, and alcohol is reported to influence serotonergic neuronal activity in the dorsal raphe. In the present study, the effects of ethanol on the synthesis of 5-HT and the expression of tryptophan hydroxylase (TPH), the rate-limiting enzyme of 5-HT synthesis, in the dorsal and median raphe of rats were investigated, using immunohistochemical methods. Results showed that the density of 5-HT-positive and TPH-positive cells was reduced by ethanol treatment in a time-dependent manner. Based on these results, it is suggested that the pathogenesis of ethanol-induced neuropsychological disorders involves ethanol-induced suppression of 5-HT synthesis and TPH expression in the raphe region.

AB - Serotonin (5-hydroxytryptamine, 5-HT) has been implicated in the pathophysiology of many neuropsychiatric disorders, and alcohol is reported to influence serotonergic neuronal activity in the dorsal raphe. In the present study, the effects of ethanol on the synthesis of 5-HT and the expression of tryptophan hydroxylase (TPH), the rate-limiting enzyme of 5-HT synthesis, in the dorsal and median raphe of rats were investigated, using immunohistochemical methods. Results showed that the density of 5-HT-positive and TPH-positive cells was reduced by ethanol treatment in a time-dependent manner. Based on these results, it is suggested that the pathogenesis of ethanol-induced neuropsychological disorders involves ethanol-induced suppression of 5-HT synthesis and TPH expression in the raphe region.

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KW - Tryptophan hydroxylase

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Time dependence of the effect of ethanol on serotonin synthesis and tryptophan hydroxylase expression in the dorsal and median raphe of young rats

Abstract

Keywords

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