Time and dose effect of transdermal nicotine on endothelial function

Virginia M Miller, W. Darrin Clouse, Britt H. Tonnessen, Umar S. Boston, Sandra R. Severson, Sue Bonde, Kevin S. Rud, Richard D. Hurt

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Nicotine patches are available as an over-the-counter medication for aid in smoking cessation. This study was designed to determine how nicotine patch therapy over time and dose ranges used in smoking cessation programs in humans affects endothelium-dependent relaxations. Dogs were treated with nicotine patches (11, 22, or 44 mg/day) for 2 and 5 wk. Circulating nicotine and oxidized products of nitric oxide (NOx) were measured. Coronary arteries were prepared for measurement of isometric force and aortic endothelial cells were prepared for measurement of mRNA or NO synthase (NOS) activity. Circulating nicotine increased with increasing concentrations of nicotine patches. After 5 wk of treatment with 22 mg/day patches, circulating NOx was reduced but NOS activity was increased. NOS mRNA was similar among groups. Only after 5 wk of treatment with 22 mg/day patches were endothelium-dependent relaxations reduced to α2-adrenergic agonists, ADP, and the calcium ionophore A-23187. These results suggest a time and biphasic dose effect of nicotine treatment on endothelium-dependent responses that may be related to bioavailability of NO. This complex relationship of duration and dose of nicotine treatment may explain, in part, discrepancies in effects of nicotine on endothelium-dependent responses.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume279
Issue number4 48-4
StatePublished - 2000

Fingerprint

Tobacco Use Cessation Products
Nicotine
Endothelium
Nitric Oxide Synthase
Smoking Cessation
Nitric Oxide
Adrenergic Agonists
Messenger RNA
Calcium Ionophores
Calcimycin
Adenosine Diphosphate
Biological Availability
Coronary Vessels
Endothelial Cells
Dogs

Keywords

  • Canine
  • Coronary artery
  • Nitric oxide
  • Nitric oxide synthase

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Miller, V. M., Clouse, W. D., Tonnessen, B. H., Boston, U. S., Severson, S. R., Bonde, S., ... Hurt, R. D. (2000). Time and dose effect of transdermal nicotine on endothelial function. American Journal of Physiology - Heart and Circulatory Physiology, 279(4 48-4).

Time and dose effect of transdermal nicotine on endothelial function. / Miller, Virginia M; Clouse, W. Darrin; Tonnessen, Britt H.; Boston, Umar S.; Severson, Sandra R.; Bonde, Sue; Rud, Kevin S.; Hurt, Richard D.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 279, No. 4 48-4, 2000.

Research output: Contribution to journalArticle

Miller, VM, Clouse, WD, Tonnessen, BH, Boston, US, Severson, SR, Bonde, S, Rud, KS & Hurt, RD 2000, 'Time and dose effect of transdermal nicotine on endothelial function', American Journal of Physiology - Heart and Circulatory Physiology, vol. 279, no. 4 48-4.
Miller, Virginia M ; Clouse, W. Darrin ; Tonnessen, Britt H. ; Boston, Umar S. ; Severson, Sandra R. ; Bonde, Sue ; Rud, Kevin S. ; Hurt, Richard D. / Time and dose effect of transdermal nicotine on endothelial function. In: American Journal of Physiology - Heart and Circulatory Physiology. 2000 ; Vol. 279, No. 4 48-4.
@article{04eb6ca300b745159c26822e129b2ce0,
title = "Time and dose effect of transdermal nicotine on endothelial function",
abstract = "Nicotine patches are available as an over-the-counter medication for aid in smoking cessation. This study was designed to determine how nicotine patch therapy over time and dose ranges used in smoking cessation programs in humans affects endothelium-dependent relaxations. Dogs were treated with nicotine patches (11, 22, or 44 mg/day) for 2 and 5 wk. Circulating nicotine and oxidized products of nitric oxide (NOx) were measured. Coronary arteries were prepared for measurement of isometric force and aortic endothelial cells were prepared for measurement of mRNA or NO synthase (NOS) activity. Circulating nicotine increased with increasing concentrations of nicotine patches. After 5 wk of treatment with 22 mg/day patches, circulating NOx was reduced but NOS activity was increased. NOS mRNA was similar among groups. Only after 5 wk of treatment with 22 mg/day patches were endothelium-dependent relaxations reduced to α2-adrenergic agonists, ADP, and the calcium ionophore A-23187. These results suggest a time and biphasic dose effect of nicotine treatment on endothelium-dependent responses that may be related to bioavailability of NO. This complex relationship of duration and dose of nicotine treatment may explain, in part, discrepancies in effects of nicotine on endothelium-dependent responses.",
keywords = "Canine, Coronary artery, Nitric oxide, Nitric oxide synthase",
author = "Miller, {Virginia M} and Clouse, {W. Darrin} and Tonnessen, {Britt H.} and Boston, {Umar S.} and Severson, {Sandra R.} and Sue Bonde and Rud, {Kevin S.} and Hurt, {Richard D.}",
year = "2000",
language = "English (US)",
volume = "279",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "4 48-4",

}

TY - JOUR

T1 - Time and dose effect of transdermal nicotine on endothelial function

AU - Miller, Virginia M

AU - Clouse, W. Darrin

AU - Tonnessen, Britt H.

AU - Boston, Umar S.

AU - Severson, Sandra R.

AU - Bonde, Sue

AU - Rud, Kevin S.

AU - Hurt, Richard D.

PY - 2000

Y1 - 2000

N2 - Nicotine patches are available as an over-the-counter medication for aid in smoking cessation. This study was designed to determine how nicotine patch therapy over time and dose ranges used in smoking cessation programs in humans affects endothelium-dependent relaxations. Dogs were treated with nicotine patches (11, 22, or 44 mg/day) for 2 and 5 wk. Circulating nicotine and oxidized products of nitric oxide (NOx) were measured. Coronary arteries were prepared for measurement of isometric force and aortic endothelial cells were prepared for measurement of mRNA or NO synthase (NOS) activity. Circulating nicotine increased with increasing concentrations of nicotine patches. After 5 wk of treatment with 22 mg/day patches, circulating NOx was reduced but NOS activity was increased. NOS mRNA was similar among groups. Only after 5 wk of treatment with 22 mg/day patches were endothelium-dependent relaxations reduced to α2-adrenergic agonists, ADP, and the calcium ionophore A-23187. These results suggest a time and biphasic dose effect of nicotine treatment on endothelium-dependent responses that may be related to bioavailability of NO. This complex relationship of duration and dose of nicotine treatment may explain, in part, discrepancies in effects of nicotine on endothelium-dependent responses.

AB - Nicotine patches are available as an over-the-counter medication for aid in smoking cessation. This study was designed to determine how nicotine patch therapy over time and dose ranges used in smoking cessation programs in humans affects endothelium-dependent relaxations. Dogs were treated with nicotine patches (11, 22, or 44 mg/day) for 2 and 5 wk. Circulating nicotine and oxidized products of nitric oxide (NOx) were measured. Coronary arteries were prepared for measurement of isometric force and aortic endothelial cells were prepared for measurement of mRNA or NO synthase (NOS) activity. Circulating nicotine increased with increasing concentrations of nicotine patches. After 5 wk of treatment with 22 mg/day patches, circulating NOx was reduced but NOS activity was increased. NOS mRNA was similar among groups. Only after 5 wk of treatment with 22 mg/day patches were endothelium-dependent relaxations reduced to α2-adrenergic agonists, ADP, and the calcium ionophore A-23187. These results suggest a time and biphasic dose effect of nicotine treatment on endothelium-dependent responses that may be related to bioavailability of NO. This complex relationship of duration and dose of nicotine treatment may explain, in part, discrepancies in effects of nicotine on endothelium-dependent responses.

KW - Canine

KW - Coronary artery

KW - Nitric oxide

KW - Nitric oxide synthase

UR - http://www.scopus.com/inward/record.url?scp=0033695723&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033695723&partnerID=8YFLogxK

M3 - Article

C2 - 11009480

AN - SCOPUS:0033695723

VL - 279

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 4 48-4

ER -