TIEG1-null tenocytes display age-dependent differences in their gene expression, adhesion, spreading and proliferation properties

Oualid Haddad, Laurie Gumez, John R. Hawse, Malayannan Subramaniam, Thomas C. Spelsberg, Sabine F. Bensamoun

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The remodeling of extracellular matrix is a crucial mechanism in tendon development and the proliferation of fibroblasts is a key factor in this process. The purpose of this study was to further elucidate the role of TIEG1 in mediating important tenocyte properties throughout the aging process. Wildtype and TIEG1 knockout tenocytes adhesion, spreading and proliferation were characterized on different substrates (fibronectin, collagen type I, gelatin and laminin) and the expression levels of various genes known to be involved with tendon development were analyzed by RT-PCR. The experiments revealed age-dependent and substrate-dependent properties for both wildtype and TIEG1 knockout tenocytes. Taken together, our results indicate an important role for TIEG1 in regulating tenocytes adhesion, spreading, and proliferation throughout the aging process. Understanding the basic mechanisms of TIEG1 in tenocytes may provide valuable information for treating multiple tendon disorders.

Original languageEnglish (US)
Pages (from-to)1726-1735
Number of pages10
JournalExperimental Cell Research
Volume317
Issue number12
DOIs
StatePublished - Jul 15 2011

Keywords

  • Adhesion
  • KLF10
  • Proliferation
  • Spreading
  • TIEG
  • Tenocytes

ASJC Scopus subject areas

  • Cell Biology

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