Thromboxane A2 receptor antagonists Inhibit endothelium-dependent contractions

Wolfgang Auch-Schwelk, Zvonimir S Katusic, Paul M. Vanhoutte

Research output: Contribution to journalArticle

178 Citations (Scopus)

Abstract

Endotheliutn-dependent contractions to acetylcholine and endothelium-independent contractions to oxygen-derived free radicals in the aorta of the spontaneously hypertensive rat (SHR) are mediated by an unidentified product of the cyclooxygenase pathway of arachidonic acid metabolism. To determine the role of thromboxane A2 (TXA2) or prostaglandin H2 (PGH2) in these contractions, rings of the thoracic aorta of SHR were suspended in organ chambers for measurement of isometric force. Acetylcholine caused endothelium-dependent contractions in quiescent rings from SHR aortas. Oxygen-derived free radicals generated with xanthine plus xanthine oxidase caused contractions in rings without endothelium. Dazoxiben (thromboxane synthetase inhibitor) did not affect contractions evoked by acetylcholine. AH 23,848, SQ 29,548, or R 68,070 (TXA2/PGH2 receptor antagonists) inhibited contractions to U 46,619 (a TXA2/ PGH2 receptor agonist), acetylcholine, and oxygen-derived free radicals. Acetylcholine stimulated the release of prostacyclin from Wistar-Kyoto (WKY) rat and SHR aortas but not the release of other prostaglandins (PGE2, PGF, TXA2). Oxygen-derived free radicals did not stimulate the release of prostaglandins from either SHR or WKY rat aortas. These results demonstrate that stimulation of TXA2/PGH2 receptors probably by PGH2 might be involved in endothelium-dependent contractions. Oxygen-derived free radicals, which might be an endothelium-derived contracting factor or factors, ultimately cause contraction by stimulation of TXA2/PGH2 receptors.

Original languageEnglish (US)
Pages (from-to)699-703
Number of pages5
JournalHypertension
Volume15
Issue number6 PART 2
StatePublished - Jun 1990

Fingerprint

Prostaglandin H2 Receptors Thromboxane A2
Inbred SHR Rats
Free Radicals
Endothelium
Prostaglandin H2
Thromboxane A2
Acetylcholine
Aorta
Oxygen
Inbred WKY Rats
ridogrel
Prostaglandins
Thromboxane-A Synthase
Histamine Agonists
Xanthine
Dinoprost
Xanthine Oxidase
Cholinergic Receptors
Epoprostenol
Prostaglandin-Endoperoxide Synthases

Keywords

  • Acetylcholine
  • Endothelium
  • Prostaglandins
  • Spontaneously hypertensive rats
  • Thromboxane

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Auch-Schwelk, W., Katusic, Z. S., & Vanhoutte, P. M. (1990). Thromboxane A2 receptor antagonists Inhibit endothelium-dependent contractions. Hypertension, 15(6 PART 2), 699-703.

Thromboxane A2 receptor antagonists Inhibit endothelium-dependent contractions. / Auch-Schwelk, Wolfgang; Katusic, Zvonimir S; Vanhoutte, Paul M.

In: Hypertension, Vol. 15, No. 6 PART 2, 06.1990, p. 699-703.

Research output: Contribution to journalArticle

Auch-Schwelk, W, Katusic, ZS & Vanhoutte, PM 1990, 'Thromboxane A2 receptor antagonists Inhibit endothelium-dependent contractions', Hypertension, vol. 15, no. 6 PART 2, pp. 699-703.
Auch-Schwelk, Wolfgang ; Katusic, Zvonimir S ; Vanhoutte, Paul M. / Thromboxane A2 receptor antagonists Inhibit endothelium-dependent contractions. In: Hypertension. 1990 ; Vol. 15, No. 6 PART 2. pp. 699-703.
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