Thrombin-induced vasospasm: Cellular signaling mechanisms

H. Jerius, A. Beal, David A Woodrum, A. Epstein, C. Brophy

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Background. In the setting of arterial injury, thrombin contributes to the hemostatic process by activating the coagulation cascade and platelets. We hypothesized that thrombin also contributes to hemostasis by inducing vasospasm. The purpose of this investigation was to characterize the cellular signaling mechanisms that modulate thrombin-induced vascular smooth muscle contractions. Methods. Contractile responses of intact bovine carotid artery smooth muscles were determined in a muscle bath. Contractile responses were correlated with phosphorylation events as determined with whole cell phosphorylation and two-dimensional gel electrophoresis and with immunoblotting of glycerol-urea or two-dimensional gels. Results. Thrombin (1 to 1000 units/ml) induced sustained vascular smooth muscle contractions of similar magnitude as the potent contractile agonist, endothelin. Thrombin- induced contractions were associated with increases in the phosphorylation of the myosin light chains (MLC20) and in the tyrosine phosphorylation of mitogen-activated protein kinase. Conclusions. These data suggest that thrombin is a potent physiologic contractile agonist that may modulate some forms of vasospasm. Thrombin-induced contractions are associated with the activation of two cellular signaling pathways, the myosin light chain kinase and the mitogen-activated protein kinase pathways.

Original languageEnglish (US)
Pages (from-to)46-50
Number of pages5
JournalSurgery
Volume123
Issue number1
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Thrombin
Phosphorylation
Muscle Contraction
Mitogen-Activated Protein Kinases
Vascular Smooth Muscle
Myosin-Light-Chain Kinase
Myosin Light Chains
Endothelins
Electrophoresis, Gel, Two-Dimensional
Hemostatics
Hemostasis
Baths
Carotid Arteries
Immunoblotting
Glycerol
Smooth Muscle
Tyrosine
Urea
Blood Platelets
Gels

ASJC Scopus subject areas

  • Surgery

Cite this

Thrombin-induced vasospasm : Cellular signaling mechanisms. / Jerius, H.; Beal, A.; Woodrum, David A; Epstein, A.; Brophy, C.

In: Surgery, Vol. 123, No. 1, 1998, p. 46-50.

Research output: Contribution to journalArticle

Jerius, H, Beal, A, Woodrum, DA, Epstein, A & Brophy, C 1998, 'Thrombin-induced vasospasm: Cellular signaling mechanisms', Surgery, vol. 123, no. 1, pp. 46-50. https://doi.org/10.1016/S0039-6060(98)70227-2
Jerius, H. ; Beal, A. ; Woodrum, David A ; Epstein, A. ; Brophy, C. / Thrombin-induced vasospasm : Cellular signaling mechanisms. In: Surgery. 1998 ; Vol. 123, No. 1. pp. 46-50.
@article{7e26b54dfc78443685378b7e8daefc38,
title = "Thrombin-induced vasospasm: Cellular signaling mechanisms",
abstract = "Background. In the setting of arterial injury, thrombin contributes to the hemostatic process by activating the coagulation cascade and platelets. We hypothesized that thrombin also contributes to hemostasis by inducing vasospasm. The purpose of this investigation was to characterize the cellular signaling mechanisms that modulate thrombin-induced vascular smooth muscle contractions. Methods. Contractile responses of intact bovine carotid artery smooth muscles were determined in a muscle bath. Contractile responses were correlated with phosphorylation events as determined with whole cell phosphorylation and two-dimensional gel electrophoresis and with immunoblotting of glycerol-urea or two-dimensional gels. Results. Thrombin (1 to 1000 units/ml) induced sustained vascular smooth muscle contractions of similar magnitude as the potent contractile agonist, endothelin. Thrombin- induced contractions were associated with increases in the phosphorylation of the myosin light chains (MLC20) and in the tyrosine phosphorylation of mitogen-activated protein kinase. Conclusions. These data suggest that thrombin is a potent physiologic contractile agonist that may modulate some forms of vasospasm. Thrombin-induced contractions are associated with the activation of two cellular signaling pathways, the myosin light chain kinase and the mitogen-activated protein kinase pathways.",
author = "H. Jerius and A. Beal and Woodrum, {David A} and A. Epstein and C. Brophy",
year = "1998",
doi = "10.1016/S0039-6060(98)70227-2",
language = "English (US)",
volume = "123",
pages = "46--50",
journal = "Surgery (United States)",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "1",

}

TY - JOUR

T1 - Thrombin-induced vasospasm

T2 - Cellular signaling mechanisms

AU - Jerius, H.

AU - Beal, A.

AU - Woodrum, David A

AU - Epstein, A.

AU - Brophy, C.

PY - 1998

Y1 - 1998

N2 - Background. In the setting of arterial injury, thrombin contributes to the hemostatic process by activating the coagulation cascade and platelets. We hypothesized that thrombin also contributes to hemostasis by inducing vasospasm. The purpose of this investigation was to characterize the cellular signaling mechanisms that modulate thrombin-induced vascular smooth muscle contractions. Methods. Contractile responses of intact bovine carotid artery smooth muscles were determined in a muscle bath. Contractile responses were correlated with phosphorylation events as determined with whole cell phosphorylation and two-dimensional gel electrophoresis and with immunoblotting of glycerol-urea or two-dimensional gels. Results. Thrombin (1 to 1000 units/ml) induced sustained vascular smooth muscle contractions of similar magnitude as the potent contractile agonist, endothelin. Thrombin- induced contractions were associated with increases in the phosphorylation of the myosin light chains (MLC20) and in the tyrosine phosphorylation of mitogen-activated protein kinase. Conclusions. These data suggest that thrombin is a potent physiologic contractile agonist that may modulate some forms of vasospasm. Thrombin-induced contractions are associated with the activation of two cellular signaling pathways, the myosin light chain kinase and the mitogen-activated protein kinase pathways.

AB - Background. In the setting of arterial injury, thrombin contributes to the hemostatic process by activating the coagulation cascade and platelets. We hypothesized that thrombin also contributes to hemostasis by inducing vasospasm. The purpose of this investigation was to characterize the cellular signaling mechanisms that modulate thrombin-induced vascular smooth muscle contractions. Methods. Contractile responses of intact bovine carotid artery smooth muscles were determined in a muscle bath. Contractile responses were correlated with phosphorylation events as determined with whole cell phosphorylation and two-dimensional gel electrophoresis and with immunoblotting of glycerol-urea or two-dimensional gels. Results. Thrombin (1 to 1000 units/ml) induced sustained vascular smooth muscle contractions of similar magnitude as the potent contractile agonist, endothelin. Thrombin- induced contractions were associated with increases in the phosphorylation of the myosin light chains (MLC20) and in the tyrosine phosphorylation of mitogen-activated protein kinase. Conclusions. These data suggest that thrombin is a potent physiologic contractile agonist that may modulate some forms of vasospasm. Thrombin-induced contractions are associated with the activation of two cellular signaling pathways, the myosin light chain kinase and the mitogen-activated protein kinase pathways.

UR - http://www.scopus.com/inward/record.url?scp=0031882267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031882267&partnerID=8YFLogxK

U2 - 10.1016/S0039-6060(98)70227-2

DO - 10.1016/S0039-6060(98)70227-2

M3 - Article

C2 - 9457222

AN - SCOPUS:0031882267

VL - 123

SP - 46

EP - 50

JO - Surgery (United States)

JF - Surgery (United States)

SN - 0039-6060

IS - 1

ER -