Thrombin expression in prostate: A novel finding

Manish Kohli; Karin Williams; Jorge L. Yao; Richard A. Dennis; Jiaoti Huang; Jay Reeder; William A. Ricke

doi:10.3109/07357907.2010.535057

Thrombin expression in prostate: A novel finding

Manish Kohli, Karin Williams, Jorge L. Yao, Richard A. Dennis, Jiaoti Huang, Jay Reeder, William A. Ricke

Medical Oncology

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Introduction: A variable repertoire of coagulation protein expression is observed in different cancers. We evaluated expression of thrombin in prostate tissue. Methods: Detection of thrombin was performed using quantitative real-time PCR in fresh tissue and in situ hybridization (ISH) in archival prostate tissue and by immunohistochemistry of prostate tissue microarrays. Results: (Pro)thrombin mRNA expression was detected in cancer tissue and localized to prostatic epithelium and stroma by ISH. Thrombin protein was detected in stroma of benign and malignant epithelium (p <.05). Conclusions: Prostate tissue is a rich reservoir of thrombin. This may have potential for developing antithrombin-based cancer therapy.

Original language	English (US)
Pages (from-to)	62-67
Number of pages	6
Journal	Cancer Investigation
Volume	29
Issue number	1
DOIs	https://doi.org/10.3109/07357907.2010.535057
State	Published - Jan 2011

Keywords

Coagulation
Prostatic neoplasms
Prothrombin

ASJC Scopus subject areas

Oncology
Cancer Research

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10.3109/07357907.2010.535057

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title = "Thrombin expression in prostate: A novel finding",

abstract = "Introduction: A variable repertoire of coagulation protein expression is observed in different cancers. We evaluated expression of thrombin in prostate tissue. Methods: Detection of thrombin was performed using quantitative real-time PCR in fresh tissue and in situ hybridization (ISH) in archival prostate tissue and by immunohistochemistry of prostate tissue microarrays. Results: (Pro)thrombin mRNA expression was detected in cancer tissue and localized to prostatic epithelium and stroma by ISH. Thrombin protein was detected in stroma of benign and malignant epithelium (p <.05). Conclusions: Prostate tissue is a rich reservoir of thrombin. This may have potential for developing antithrombin-based cancer therapy.",

keywords = "Coagulation, Prostatic neoplasms, Prothrombin",

author = "Manish Kohli and Karin Williams and Yao, {Jorge L.} and Dennis, {Richard A.} and Jiaoti Huang and Jay Reeder and Ricke, {William A.}",

note = "Funding Information: The study was supported by Departmental funds from Medicine and Urology, University of Rochester, NCI grant no. CA091956 (Prostate SPORE Awarded to Mayo Clinic), and Funding source, CA123199 (WAR).",

year = "2011",

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doi = "10.3109/07357907.2010.535057",

language = "English (US)",

volume = "29",

pages = "62--67",

journal = "Cancer Investigation",

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TY - JOUR

T1 - Thrombin expression in prostate

T2 - A novel finding

AU - Kohli, Manish

AU - Williams, Karin

AU - Yao, Jorge L.

AU - Dennis, Richard A.

AU - Huang, Jiaoti

AU - Reeder, Jay

AU - Ricke, William A.

N1 - Funding Information: The study was supported by Departmental funds from Medicine and Urology, University of Rochester, NCI grant no. CA091956 (Prostate SPORE Awarded to Mayo Clinic), and Funding source, CA123199 (WAR).

PY - 2011/1

Y1 - 2011/1

N2 - Introduction: A variable repertoire of coagulation protein expression is observed in different cancers. We evaluated expression of thrombin in prostate tissue. Methods: Detection of thrombin was performed using quantitative real-time PCR in fresh tissue and in situ hybridization (ISH) in archival prostate tissue and by immunohistochemistry of prostate tissue microarrays. Results: (Pro)thrombin mRNA expression was detected in cancer tissue and localized to prostatic epithelium and stroma by ISH. Thrombin protein was detected in stroma of benign and malignant epithelium (p <.05). Conclusions: Prostate tissue is a rich reservoir of thrombin. This may have potential for developing antithrombin-based cancer therapy.

AB - Introduction: A variable repertoire of coagulation protein expression is observed in different cancers. We evaluated expression of thrombin in prostate tissue. Methods: Detection of thrombin was performed using quantitative real-time PCR in fresh tissue and in situ hybridization (ISH) in archival prostate tissue and by immunohistochemistry of prostate tissue microarrays. Results: (Pro)thrombin mRNA expression was detected in cancer tissue and localized to prostatic epithelium and stroma by ISH. Thrombin protein was detected in stroma of benign and malignant epithelium (p <.05). Conclusions: Prostate tissue is a rich reservoir of thrombin. This may have potential for developing antithrombin-based cancer therapy.

KW - Coagulation

KW - Prostatic neoplasms

KW - Prothrombin

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JO - Cancer Investigation

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Thrombin expression in prostate: A novel finding

Abstract

Keywords

ASJC Scopus subject areas

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