Abstract
Introduction: A variable repertoire of coagulation protein expression is observed in different cancers. We evaluated expression of thrombin in prostate tissue. Methods: Detection of thrombin was performed using quantitative real-time PCR in fresh tissue and in situ hybridization (ISH) in archival prostate tissue and by immunohistochemistry of prostate tissue microarrays. Results: (Pro)thrombin mRNA expression was detected in cancer tissue and localized to prostatic epithelium and stroma by ISH. Thrombin protein was detected in stroma of benign and malignant epithelium (p <.05). Conclusions: Prostate tissue is a rich reservoir of thrombin. This may have potential for developing antithrombin-based cancer therapy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 62-67 |
| Number of pages | 6 |
| Journal | Cancer Investigation |
| Volume | 29 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2011 |
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Keywords
- Coagulation
- Prostatic neoplasms
- Prothrombin
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Thrombin expression in prostate : A novel finding. / Kohli, Manish; Williams, Karin; Yao, Jorge L.; Dennis, Richard A.; Huang, Jiaoti; Reeder, Jay; Ricke, William A.
In: Cancer Investigation, Vol. 29, No. 1, 01.2011, p. 62-67.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Thrombin expression in prostate
T2 - A novel finding
AU - Kohli, Manish
AU - Williams, Karin
AU - Yao, Jorge L.
AU - Dennis, Richard A.
AU - Huang, Jiaoti
AU - Reeder, Jay
AU - Ricke, William A.
PY - 2011/1
Y1 - 2011/1
N2 - Introduction: A variable repertoire of coagulation protein expression is observed in different cancers. We evaluated expression of thrombin in prostate tissue. Methods: Detection of thrombin was performed using quantitative real-time PCR in fresh tissue and in situ hybridization (ISH) in archival prostate tissue and by immunohistochemistry of prostate tissue microarrays. Results: (Pro)thrombin mRNA expression was detected in cancer tissue and localized to prostatic epithelium and stroma by ISH. Thrombin protein was detected in stroma of benign and malignant epithelium (p <.05). Conclusions: Prostate tissue is a rich reservoir of thrombin. This may have potential for developing antithrombin-based cancer therapy.
AB - Introduction: A variable repertoire of coagulation protein expression is observed in different cancers. We evaluated expression of thrombin in prostate tissue. Methods: Detection of thrombin was performed using quantitative real-time PCR in fresh tissue and in situ hybridization (ISH) in archival prostate tissue and by immunohistochemistry of prostate tissue microarrays. Results: (Pro)thrombin mRNA expression was detected in cancer tissue and localized to prostatic epithelium and stroma by ISH. Thrombin protein was detected in stroma of benign and malignant epithelium (p <.05). Conclusions: Prostate tissue is a rich reservoir of thrombin. This may have potential for developing antithrombin-based cancer therapy.
KW - Coagulation
KW - Prostatic neoplasms
KW - Prothrombin
UR - http://www.scopus.com/inward/record.url?scp=78650499724&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78650499724&partnerID=8YFLogxK
U2 - 10.3109/07357907.2010.535057
DO - 10.3109/07357907.2010.535057
M3 - Article
C2 - 21166500
AN - SCOPUS:78650499724
VL - 29
SP - 62
EP - 67
JO - Cancer Investigation
JF - Cancer Investigation
SN - 0735-7907
IS - 1
ER -