Thrombin expression in prostate: A novel finding

Manish Kohli, Karin Williams, Jorge L. Yao, Richard A. Dennis, Jiaoti Huang, Jay Reeder, William A. Ricke

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Introduction: A variable repertoire of coagulation protein expression is observed in different cancers. We evaluated expression of thrombin in prostate tissue. Methods: Detection of thrombin was performed using quantitative real-time PCR in fresh tissue and in situ hybridization (ISH) in archival prostate tissue and by immunohistochemistry of prostate tissue microarrays. Results: (Pro)thrombin mRNA expression was detected in cancer tissue and localized to prostatic epithelium and stroma by ISH. Thrombin protein was detected in stroma of benign and malignant epithelium (p <.05). Conclusions: Prostate tissue is a rich reservoir of thrombin. This may have potential for developing antithrombin-based cancer therapy.

Original languageEnglish (US)
Pages (from-to)62-67
Number of pages6
JournalCancer Investigation
Volume29
Issue number1
DOIs
StatePublished - Jan 2011

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Thrombin
Prostate
In Situ Hybridization
Epithelium
Neoplasms
Antithrombins
Real-Time Polymerase Chain Reaction
Proteins
Immunohistochemistry
Messenger RNA

Keywords

  • Coagulation
  • Prostatic neoplasms
  • Prothrombin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Kohli, M., Williams, K., Yao, J. L., Dennis, R. A., Huang, J., Reeder, J., & Ricke, W. A. (2011). Thrombin expression in prostate: A novel finding. Cancer Investigation, 29(1), 62-67. https://doi.org/10.3109/07357907.2010.535057

Thrombin expression in prostate : A novel finding. / Kohli, Manish; Williams, Karin; Yao, Jorge L.; Dennis, Richard A.; Huang, Jiaoti; Reeder, Jay; Ricke, William A.

In: Cancer Investigation, Vol. 29, No. 1, 01.2011, p. 62-67.

Research output: Contribution to journalArticle

Kohli, M, Williams, K, Yao, JL, Dennis, RA, Huang, J, Reeder, J & Ricke, WA 2011, 'Thrombin expression in prostate: A novel finding', Cancer Investigation, vol. 29, no. 1, pp. 62-67. https://doi.org/10.3109/07357907.2010.535057
Kohli M, Williams K, Yao JL, Dennis RA, Huang J, Reeder J et al. Thrombin expression in prostate: A novel finding. Cancer Investigation. 2011 Jan;29(1):62-67. https://doi.org/10.3109/07357907.2010.535057
Kohli, Manish ; Williams, Karin ; Yao, Jorge L. ; Dennis, Richard A. ; Huang, Jiaoti ; Reeder, Jay ; Ricke, William A. / Thrombin expression in prostate : A novel finding. In: Cancer Investigation. 2011 ; Vol. 29, No. 1. pp. 62-67.
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