Three-dimensional structure of neurons from peripheral autonomic ganglia using confocal microscope images

Steven M. Miller, Philip Schmalz, Leonid Ermilov, James Tarara, Ruchun Ma, James Brewer, Joseph H. Szurszewski

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

An understanding of neuronal physiology at the single unit level requires knowledge of the functional properties of the neurons as well as a detailed description of its morphology. A full morphological description should include a three-dimensional (3-D) analysis. In this paper, we describe for the first time, 3-D morphology of individual neurons of the peripheral autonomic nervous system intact within whole-mount preparations. Neurons were filled with the fluorescent dye Lucifer Yellow and imaged by laser scanning confocal microscopy. Three- dimensional reconstructions were obtained using volume rendering methods on a set of serial optical sections obtained for each neuron. The 3-D images of the neurons we studied showed a complexity of shape and detail that is not readily apparent from images viewed by traditional microscopy.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages640-650
Number of pages11
ISBN (Print)081941008X
StatePublished - 1992
EventVisualization in Biomedical Computing '92 - Chapel Hill, NC, USA
Duration: Oct 13 1992Oct 16 1992

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1808
ISSN (Print)0277-786X

Other

OtherVisualization in Biomedical Computing '92
CityChapel Hill, NC, USA
Period10/13/9210/16/92

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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