TY - GEN
T1 - Three-dimensional structure of neurons from peripheral autonomic ganglia using confocal microscope images
AU - Miller, Steven M.
AU - Schmalz, Philip
AU - Ermilov, Leonid
AU - Tarara, James
AU - Ma, Ruchun
AU - Brewer, James
AU - Szurszewski, Joseph H.
PY - 1992
Y1 - 1992
N2 - An understanding of neuronal physiology at the single unit level requires knowledge of the functional properties of the neurons as well as a detailed description of its morphology. A full morphological description should include a three-dimensional (3-D) analysis. In this paper, we describe for the first time, 3-D morphology of individual neurons of the peripheral autonomic nervous system intact within whole-mount preparations. Neurons were filled with the fluorescent dye Lucifer Yellow and imaged by laser scanning confocal microscopy. Three- dimensional reconstructions were obtained using volume rendering methods on a set of serial optical sections obtained for each neuron. The 3-D images of the neurons we studied showed a complexity of shape and detail that is not readily apparent from images viewed by traditional microscopy.
AB - An understanding of neuronal physiology at the single unit level requires knowledge of the functional properties of the neurons as well as a detailed description of its morphology. A full morphological description should include a three-dimensional (3-D) analysis. In this paper, we describe for the first time, 3-D morphology of individual neurons of the peripheral autonomic nervous system intact within whole-mount preparations. Neurons were filled with the fluorescent dye Lucifer Yellow and imaged by laser scanning confocal microscopy. Three- dimensional reconstructions were obtained using volume rendering methods on a set of serial optical sections obtained for each neuron. The 3-D images of the neurons we studied showed a complexity of shape and detail that is not readily apparent from images viewed by traditional microscopy.
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M3 - Conference contribution
AN - SCOPUS:0026981032
SN - 081941008X
T3 - Proceedings of SPIE - The International Society for Optical Engineering
SP - 640
EP - 650
BT - Proceedings of SPIE - The International Society for Optical Engineering
PB - Publ by Int Soc for Optical Engineering
T2 - Visualization in Biomedical Computing '92
Y2 - 13 October 1992 through 16 October 1992
ER -