Three-dimensional microcomputed tomography of renal vasculature in rats

Agustin Garcia-Sanz, Alicia Rodriguez-Barbero, Michael D. Bentley, Erik L. Ritman, J. Carlos Romero

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Current microscopic methods to view renal microvasculature reveal only a very limited portion of the total renal volume. Identification of connectivity for postglomerular vessels in the cortex and the medulla during functional states related to changes in sodium excretion will help better to understand the coupling of renal vasculature to tubular function. The purpose of this study was to investigate the possibility of visualizing the continuity of pre- and postglomerular vasculature using three-dimensional micro-computed tomography (micro-CT). Kidneys from normal rats were perfusion fixed in situ at physiological pressure, filled with latex microfilm containing lead chromate, and embedded in plastic. The micro-CT scans of the intact kidneys were carried out on a rotating stage illuminated either by a synchrotron x-ray source or a conventional x-ray spectroscopy tube. Images were reconstructed by a filtered back projection algorithm and volume- rendering techniques were utilized to display the vasculature. The reconstructed images clearly showed the large distribution vessels and the venous drainage of the kidneys, while pre- and postglomerular vessels and their vascular connections throughout the kidney were displayed in greater detail. Efferent arterioles showed the characteristics of their peritubular capillary beds in the cortical and medullary regions. The vascular volume of the cortex was 27%, the outer stripe of the outermedulla 18%, the inner stripe of the outer medulla 30%, and the inner medulla 18%. In conclusion, micro-CT is a promising method to evaluate renal vascular architecture relative to physiological and pathological alterations.

Original languageEnglish (US)
Pages (from-to)440-444
Number of pages5
JournalHypertension
Volume31
Issue number1 II SUPPL.
StatePublished - Jan 1998

Fingerprint

X-Ray Microtomography
Kidney
Blood Vessels
Tomography
X-Rays
Synchrotrons
Latex
Arterioles
Microvessels
Plastics
Drainage
Spectrum Analysis
Perfusion
Sodium
Pressure

Keywords

  • Capillaries
  • Imaging
  • Kidney
  • Microcirculation
  • Tomography
  • Vasculature

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Garcia-Sanz, A., Rodriguez-Barbero, A., Bentley, M. D., Ritman, E. L., & Romero, J. C. (1998). Three-dimensional microcomputed tomography of renal vasculature in rats. Hypertension, 31(1 II SUPPL.), 440-444.

Three-dimensional microcomputed tomography of renal vasculature in rats. / Garcia-Sanz, Agustin; Rodriguez-Barbero, Alicia; Bentley, Michael D.; Ritman, Erik L.; Romero, J. Carlos.

In: Hypertension, Vol. 31, No. 1 II SUPPL., 01.1998, p. 440-444.

Research output: Contribution to journalArticle

Garcia-Sanz, A, Rodriguez-Barbero, A, Bentley, MD, Ritman, EL & Romero, JC 1998, 'Three-dimensional microcomputed tomography of renal vasculature in rats', Hypertension, vol. 31, no. 1 II SUPPL., pp. 440-444.
Garcia-Sanz A, Rodriguez-Barbero A, Bentley MD, Ritman EL, Romero JC. Three-dimensional microcomputed tomography of renal vasculature in rats. Hypertension. 1998 Jan;31(1 II SUPPL.):440-444.
Garcia-Sanz, Agustin ; Rodriguez-Barbero, Alicia ; Bentley, Michael D. ; Ritman, Erik L. ; Romero, J. Carlos. / Three-dimensional microcomputed tomography of renal vasculature in rats. In: Hypertension. 1998 ; Vol. 31, No. 1 II SUPPL. pp. 440-444.
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