Three conserved transcriptional repressor domains are a defining feature of the TIEG subfamily of Sp1-like zinc finger proteins

Tiffany Cook, Brian Gebelein, Mohammad Belal, Kristin Mesa, Raul Urrutia

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Sp1-like transcription factors are characterized by three highly homologous C-terminal zinc finger motifs that bind GC-rich sequences. These proteins behave as either activators or repressors and have begun to be classified into different subfamilies based upon the presence of conserved motifs outside the zinc finger domain. This classification predicts that different Sp1-like subfamilies share certain functional properties. TIEG1 and TIEG2 constitute a new subfamily of transforming growth factor-β-inducible Sp1-like proteins whose zinc finger motifs also bind GC-rich sequences. However, regions outside of the DNA-binding domain that differ in structure from other Sp1-like family members remain poorly characterized. Here, we have used extensive mutagenesis and GAL4-based transcriptional assays to identify three repression domains within TIEG1 and TIEG2 that we call R1, R2, and R3. R1 is 10 amino acids, R2 is 12 amino acids, and R3 is approximately 80 amino acids long. None of these domains share homology with previously described transcriptional regulatory motifs, but they share strong sequence boo mology and are functionally conserved between TIEG1 and TIEG2. Together, these data demonstrate that TIEG proteins are capable of repressing transcription, define domains critical for this function, and further support the idea that different subfamilies of Sp1-like proteins have evolved to mediate distinct transcriptional functions.

Original languageEnglish (US)
Pages (from-to)29500-29504
Number of pages5
JournalJournal of Biological Chemistry
Volume274
Issue number41
DOIs
StatePublished - Oct 8 1999

Fingerprint

Zinc Fingers
Zinc
GC Rich Sequence
Amino Acids
Proteins
Sp1 Transcription Factor
Mutagenesis
Transforming Growth Factors
Transcription
Assays
Transcription Factors
DNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Three conserved transcriptional repressor domains are a defining feature of the TIEG subfamily of Sp1-like zinc finger proteins. / Cook, Tiffany; Gebelein, Brian; Belal, Mohammad; Mesa, Kristin; Urrutia, Raul.

In: Journal of Biological Chemistry, Vol. 274, No. 41, 08.10.1999, p. 29500-29504.

Research output: Contribution to journalArticle

Cook, Tiffany ; Gebelein, Brian ; Belal, Mohammad ; Mesa, Kristin ; Urrutia, Raul. / Three conserved transcriptional repressor domains are a defining feature of the TIEG subfamily of Sp1-like zinc finger proteins. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 41. pp. 29500-29504.
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