Thermal preconditioning and heat-shock protein 72 preserve synaptic transmission during thermal stress.

Jonathan D. Kelty, Peter Noseworthy, Martin E. Feder, R. Meldrum Robertson, Jan Marino Ramirez

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

As with other tissues, exposing the mammalian CNS to nonlethal heat stress (i.e., thermal preconditioning) increases levels of heat-shock proteins (Hsps) such as Hsp70 and enhances the viability of neurons under subsequent stress. Using a medullary slice preparation from a neonatal mouse, including the site of the neural network that generates respiratory rhythm (the pre-Bötzinger complex), we show that thermal preconditioning has an additional fundamental effect, protection of synaptic function. Relative to 30 degrees C baseline, initial thermal stress (40 degrees C) greatly increased the frequency of synaptic currents recorded without pharmacological manipulation by approximately 17-fold (p < 0.01) and of miniature postsynaptic currents (mPSCs) elicited by GABA (20-fold) glutamate (10-fold), and glycine (36-fold). Thermal preconditioning (15 min at 40 degrees C) eliminated the increase in frequency of overall synaptic transmission during acute thermal stress and greatly attenuated the frequency increases of GABAergic, glutamatergic, and glycinergic mPSCs (for each, p < 0.05). Moreover, without thermal preconditioning, incubation of slices in solution containing inducible Hsp70 (Hsp72) mimicked the effect of thermal preconditioning on the stress-induced release of neurotransmitter. That preconditioning and exogenous Hsp72 can affect and preserve normal physiological function has important therapeutic implications.

Original languageEnglish (US)
JournalThe Journal of neuroscience : the official journal of the Society for Neuroscience
Volume22
Issue number1
StatePublished - Jan 1 2002
Externally publishedYes

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HSP72 Heat-Shock Proteins
Synaptic Transmission
Hot Temperature
Synaptic Potentials
Heat-Shock Proteins
Glycine
gamma-Aminobutyric Acid
Neurotransmitter Agents
Glutamic Acid
Pharmacology
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Thermal preconditioning and heat-shock protein 72 preserve synaptic transmission during thermal stress. / Kelty, Jonathan D.; Noseworthy, Peter; Feder, Martin E.; Robertson, R. Meldrum; Ramirez, Jan Marino.

In: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 22, No. 1, 01.01.2002.

Research output: Contribution to journalArticle

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