Therapeutic efficacy of adoptive immunotherapy is predicated on in vivo antigen-specific proliferation of donor T cells

Jørgen Kjaergaard, Liaomin Peng, Peter A. Cohen, Suyu Shu

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Activated T cells with down-regulated L-selectin expression (L-sel-) from tumor-draining lymph nodes represent a potent source of specific immune effectors in adoptive immunotherapy. Using congenic pairs of mice and carboxyfluorescein diacetate succinimidyl ester-labeled L-sel- T cells, the current study analyzed in vivo proliferation of transferred cells. In the lung of MCA205 tumor-bearing mice, 6% or 0.3 × 106 of the 5 × 106 donor cells were identified 24 h after transfer. Vigorous proliferation of donor cells was evident on day 2, reaching a maximum on day 6. The proliferation was tumor-specific and CD4 T cells divided with greater magnitude than CD8 cells. Successful adoptive immunotherapy also required sublethal whole-body irradiation (WBI) of the recipient. WBI exerted its effects on facilitating specific T cell proliferation at the tumor site. Taken together, our results demonstrate that adoptively transferred T cells undergo extensive proliferation in response to the tumor and this response is associated with therapeutic efficacy.

Original languageEnglish (US)
Pages (from-to)8-20
Number of pages13
JournalClinical Immunology
Volume108
Issue number1
DOIs
StatePublished - Jul 1 2003

Keywords

  • Adoptive immunotherapy
  • L-selectin
  • T lymphocytes
  • Tumor-draining lymph nodes
  • Whole-body irradiation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Therapeutic efficacy of adoptive immunotherapy is predicated on in vivo antigen-specific proliferation of donor T cells'. Together they form a unique fingerprint.

  • Cite this