Abstract
Activated T cells with down-regulated L-selectin expression (L-sel-) from tumor-draining lymph nodes represent a potent source of specific immune effectors in adoptive immunotherapy. Using congenic pairs of mice and carboxyfluorescein diacetate succinimidyl ester-labeled L-sel- T cells, the current study analyzed in vivo proliferation of transferred cells. In the lung of MCA205 tumor-bearing mice, 6% or 0.3 × 106 of the 5 × 106 donor cells were identified 24 h after transfer. Vigorous proliferation of donor cells was evident on day 2, reaching a maximum on day 6. The proliferation was tumor-specific and CD4 T cells divided with greater magnitude than CD8 cells. Successful adoptive immunotherapy also required sublethal whole-body irradiation (WBI) of the recipient. WBI exerted its effects on facilitating specific T cell proliferation at the tumor site. Taken together, our results demonstrate that adoptively transferred T cells undergo extensive proliferation in response to the tumor and this response is associated with therapeutic efficacy.
Original language | English (US) |
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Pages (from-to) | 8-20 |
Number of pages | 13 |
Journal | Clinical Immunology |
Volume | 108 |
Issue number | 1 |
DOIs | |
State | Published - Jul 1 2003 |
Keywords
- Adoptive immunotherapy
- L-selectin
- T lymphocytes
- Tumor-draining lymph nodes
- Whole-body irradiation
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology