Theory and applications of the polymerase chain reaction

D. G. Remick, S. L. Kunkel, E. A. Holbrook, C. A. Hanson

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The polymerase chain reaction (PCR) is a newly developed molecular biology technique that can significantly amplify DNA or RNA. The process consists of repetitive cycles of specific DNA synthesis, defined by short stretches of preselected DNA. With each cycle, there is a doubling of the final, desired DNA product such that a million-fold amplification is possible. This powerful method has numerous applications in diagnostic pathology, especially in the fields of microbiology, forensic science, and hematology. The PCR may be used to directly detect viral DNA, which may facilitate the diagnosis of acquired immune deficiency syndrome (AIDS) or other viral diseases. PCR amplification of DNA allows detection of specific sequences from extremely small samples, such as with forensic material. In hematology, PCR may help in the diagnosis of hemoglobinopathies or of neoplastic disorders by documenting chromosomal translocations. The new PCR opens exciting new avenues for diagnostic pathology using this new technology.

Original languageEnglish (US)
JournalAmerican Journal of Clinical Pathology
Volume93
Issue number4 SUPPL. 1
StatePublished - 1990
Externally publishedYes

Fingerprint

Polymerase Chain Reaction
DNA
Hematology
Pathology
Forensic Sciences
Hemoglobinopathies
Genetic Translocation
Viral DNA
Virus Diseases
Microbiology
Molecular Biology
Acquired Immunodeficiency Syndrome
RNA
Technology

Keywords

  • AIDS
  • Diagnostic pathology
  • Forensic pathology
  • Gene amplification
  • Hematology
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Remick, D. G., Kunkel, S. L., Holbrook, E. A., & Hanson, C. A. (1990). Theory and applications of the polymerase chain reaction. American Journal of Clinical Pathology, 93(4 SUPPL. 1).

Theory and applications of the polymerase chain reaction. / Remick, D. G.; Kunkel, S. L.; Holbrook, E. A.; Hanson, C. A.

In: American Journal of Clinical Pathology, Vol. 93, No. 4 SUPPL. 1, 1990.

Research output: Contribution to journalArticle

Remick, DG, Kunkel, SL, Holbrook, EA & Hanson, CA 1990, 'Theory and applications of the polymerase chain reaction', American Journal of Clinical Pathology, vol. 93, no. 4 SUPPL. 1.
Remick DG, Kunkel SL, Holbrook EA, Hanson CA. Theory and applications of the polymerase chain reaction. American Journal of Clinical Pathology. 1990;93(4 SUPPL. 1).
Remick, D. G. ; Kunkel, S. L. ; Holbrook, E. A. ; Hanson, C. A. / Theory and applications of the polymerase chain reaction. In: American Journal of Clinical Pathology. 1990 ; Vol. 93, No. 4 SUPPL. 1.
@article{bf41d804394446118f09f0dc87a4e6ca,
title = "Theory and applications of the polymerase chain reaction",
abstract = "The polymerase chain reaction (PCR) is a newly developed molecular biology technique that can significantly amplify DNA or RNA. The process consists of repetitive cycles of specific DNA synthesis, defined by short stretches of preselected DNA. With each cycle, there is a doubling of the final, desired DNA product such that a million-fold amplification is possible. This powerful method has numerous applications in diagnostic pathology, especially in the fields of microbiology, forensic science, and hematology. The PCR may be used to directly detect viral DNA, which may facilitate the diagnosis of acquired immune deficiency syndrome (AIDS) or other viral diseases. PCR amplification of DNA allows detection of specific sequences from extremely small samples, such as with forensic material. In hematology, PCR may help in the diagnosis of hemoglobinopathies or of neoplastic disorders by documenting chromosomal translocations. The new PCR opens exciting new avenues for diagnostic pathology using this new technology.",
keywords = "AIDS, Diagnostic pathology, Forensic pathology, Gene amplification, Hematology, Polymerase chain reaction",
author = "Remick, {D. G.} and Kunkel, {S. L.} and Holbrook, {E. A.} and Hanson, {C. A.}",
year = "1990",
language = "English (US)",
volume = "93",
journal = "American Journal of Clinical Pathology",
issn = "0002-9173",
publisher = "American Society of Clinical Pathologists",
number = "4 SUPPL. 1",

}

TY - JOUR

T1 - Theory and applications of the polymerase chain reaction

AU - Remick, D. G.

AU - Kunkel, S. L.

AU - Holbrook, E. A.

AU - Hanson, C. A.

PY - 1990

Y1 - 1990

N2 - The polymerase chain reaction (PCR) is a newly developed molecular biology technique that can significantly amplify DNA or RNA. The process consists of repetitive cycles of specific DNA synthesis, defined by short stretches of preselected DNA. With each cycle, there is a doubling of the final, desired DNA product such that a million-fold amplification is possible. This powerful method has numerous applications in diagnostic pathology, especially in the fields of microbiology, forensic science, and hematology. The PCR may be used to directly detect viral DNA, which may facilitate the diagnosis of acquired immune deficiency syndrome (AIDS) or other viral diseases. PCR amplification of DNA allows detection of specific sequences from extremely small samples, such as with forensic material. In hematology, PCR may help in the diagnosis of hemoglobinopathies or of neoplastic disorders by documenting chromosomal translocations. The new PCR opens exciting new avenues for diagnostic pathology using this new technology.

AB - The polymerase chain reaction (PCR) is a newly developed molecular biology technique that can significantly amplify DNA or RNA. The process consists of repetitive cycles of specific DNA synthesis, defined by short stretches of preselected DNA. With each cycle, there is a doubling of the final, desired DNA product such that a million-fold amplification is possible. This powerful method has numerous applications in diagnostic pathology, especially in the fields of microbiology, forensic science, and hematology. The PCR may be used to directly detect viral DNA, which may facilitate the diagnosis of acquired immune deficiency syndrome (AIDS) or other viral diseases. PCR amplification of DNA allows detection of specific sequences from extremely small samples, such as with forensic material. In hematology, PCR may help in the diagnosis of hemoglobinopathies or of neoplastic disorders by documenting chromosomal translocations. The new PCR opens exciting new avenues for diagnostic pathology using this new technology.

KW - AIDS

KW - Diagnostic pathology

KW - Forensic pathology

KW - Gene amplification

KW - Hematology

KW - Polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=0025321324&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025321324&partnerID=8YFLogxK

M3 - Article

C2 - 2180280

AN - SCOPUS:0025321324

VL - 93

JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

SN - 0002-9173

IS - 4 SUPPL. 1

ER -