Theiler's virus-infected L-selectin-deficient mice have decreased infiltration of CD8+ T lymphocytes in central nervous system but clear the virus

Xuexian Zhang, Laurie Brewer, Bruce Walcheck, Aaron J. Johnson, Larry R Pease, M. Kariuki Njenga

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Mice with targeted deletion of L-selectin gene (L-sel-/-) were used to investigate the role of adhesion molecule in immunologic responses following virus infection in the central nervous system (CNS). L-Sel-/- mice from a resistant H-2b genetic background and parental wild-type H-2b (C57BL/6) mice were infected with Theiler's murine encephalomyelitis virus (TMEV) intracerebrally and the kinetics of virus replication and infiltration of immune cells in the CNS determined. The levels of infectious TMEV, as measured by plaque assay at 3, 7, 14, and 28 days after infection were between 4 and 6 log10 PFU of virus per gram of CNS tissues at days 3 and 7 post-infection, and then decreased to undetectable levels by day 14 after infection in both strains of mice. The L-sel-/- mice had decreased numbers of CD8+ T lymphocytes (17.72% ± 2.4) infiltrating into the CNS at 7 days post-infection when compared to wild-type mice (31.02% ± 7.5). In addition, the L-sel-/- mice had significantly lower levels of TMEV-specific serum IgG resulting in lower virus neutralizing activity of the serum when compared to wild-type mice. However, the L-sel-/- mice had 2.5-fold increase in B lymphocytes in the CNS (8.29% ± 1.1) when compared to wild-type mice (3.2% ± 0.4). Taken together, these data indicate that L-selectin plays a role in recruitment of B and CD8+ T lymphocytes into the CNS following virus infection, which, however, did not affect the ability of the mice to clear TMEV infection.

Original languageEnglish (US)
Pages (from-to)178-187
Number of pages10
JournalJournal of Neuroimmunology
Volume116
Issue number2
DOIs
StatePublished - Jun 1 2001

Fingerprint

Theilovirus
L-Selectin
Central Nervous System
Viruses
T-Lymphocytes
Virus Diseases
Infection
Nerve Tissue
Virus Replication
Serum
Inbred C57BL Mouse
B-Lymphocytes
Immunoglobulin G

Keywords

  • Central nervous system
  • Demyelination
  • L-selectin
  • Lymphocytes
  • Theiler's virus

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

Cite this

Theiler's virus-infected L-selectin-deficient mice have decreased infiltration of CD8+ T lymphocytes in central nervous system but clear the virus. / Zhang, Xuexian; Brewer, Laurie; Walcheck, Bruce; Johnson, Aaron J.; Pease, Larry R; Njenga, M. Kariuki.

In: Journal of Neuroimmunology, Vol. 116, No. 2, 01.06.2001, p. 178-187.

Research output: Contribution to journalArticle

@article{04f2777825ee47d482efb77f3bbdd96d,
title = "Theiler's virus-infected L-selectin-deficient mice have decreased infiltration of CD8+ T lymphocytes in central nervous system but clear the virus",
abstract = "Mice with targeted deletion of L-selectin gene (L-sel-/-) were used to investigate the role of adhesion molecule in immunologic responses following virus infection in the central nervous system (CNS). L-Sel-/- mice from a resistant H-2b genetic background and parental wild-type H-2b (C57BL/6) mice were infected with Theiler's murine encephalomyelitis virus (TMEV) intracerebrally and the kinetics of virus replication and infiltration of immune cells in the CNS determined. The levels of infectious TMEV, as measured by plaque assay at 3, 7, 14, and 28 days after infection were between 4 and 6 log10 PFU of virus per gram of CNS tissues at days 3 and 7 post-infection, and then decreased to undetectable levels by day 14 after infection in both strains of mice. The L-sel-/- mice had decreased numbers of CD8+ T lymphocytes (17.72{\%} ± 2.4) infiltrating into the CNS at 7 days post-infection when compared to wild-type mice (31.02{\%} ± 7.5). In addition, the L-sel-/- mice had significantly lower levels of TMEV-specific serum IgG resulting in lower virus neutralizing activity of the serum when compared to wild-type mice. However, the L-sel-/- mice had 2.5-fold increase in B lymphocytes in the CNS (8.29{\%} ± 1.1) when compared to wild-type mice (3.2{\%} ± 0.4). Taken together, these data indicate that L-selectin plays a role in recruitment of B and CD8+ T lymphocytes into the CNS following virus infection, which, however, did not affect the ability of the mice to clear TMEV infection.",
keywords = "Central nervous system, Demyelination, L-selectin, Lymphocytes, Theiler's virus",
author = "Xuexian Zhang and Laurie Brewer and Bruce Walcheck and Johnson, {Aaron J.} and Pease, {Larry R} and Njenga, {M. Kariuki}",
year = "2001",
month = "6",
day = "1",
doi = "10.1016/S0165-5728(01)00296-X",
language = "English (US)",
volume = "116",
pages = "178--187",
journal = "Journal of Neuroimmunology",
issn = "0165-5728",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Theiler's virus-infected L-selectin-deficient mice have decreased infiltration of CD8+ T lymphocytes in central nervous system but clear the virus

AU - Zhang, Xuexian

AU - Brewer, Laurie

AU - Walcheck, Bruce

AU - Johnson, Aaron J.

AU - Pease, Larry R

AU - Njenga, M. Kariuki

PY - 2001/6/1

Y1 - 2001/6/1

N2 - Mice with targeted deletion of L-selectin gene (L-sel-/-) were used to investigate the role of adhesion molecule in immunologic responses following virus infection in the central nervous system (CNS). L-Sel-/- mice from a resistant H-2b genetic background and parental wild-type H-2b (C57BL/6) mice were infected with Theiler's murine encephalomyelitis virus (TMEV) intracerebrally and the kinetics of virus replication and infiltration of immune cells in the CNS determined. The levels of infectious TMEV, as measured by plaque assay at 3, 7, 14, and 28 days after infection were between 4 and 6 log10 PFU of virus per gram of CNS tissues at days 3 and 7 post-infection, and then decreased to undetectable levels by day 14 after infection in both strains of mice. The L-sel-/- mice had decreased numbers of CD8+ T lymphocytes (17.72% ± 2.4) infiltrating into the CNS at 7 days post-infection when compared to wild-type mice (31.02% ± 7.5). In addition, the L-sel-/- mice had significantly lower levels of TMEV-specific serum IgG resulting in lower virus neutralizing activity of the serum when compared to wild-type mice. However, the L-sel-/- mice had 2.5-fold increase in B lymphocytes in the CNS (8.29% ± 1.1) when compared to wild-type mice (3.2% ± 0.4). Taken together, these data indicate that L-selectin plays a role in recruitment of B and CD8+ T lymphocytes into the CNS following virus infection, which, however, did not affect the ability of the mice to clear TMEV infection.

AB - Mice with targeted deletion of L-selectin gene (L-sel-/-) were used to investigate the role of adhesion molecule in immunologic responses following virus infection in the central nervous system (CNS). L-Sel-/- mice from a resistant H-2b genetic background and parental wild-type H-2b (C57BL/6) mice were infected with Theiler's murine encephalomyelitis virus (TMEV) intracerebrally and the kinetics of virus replication and infiltration of immune cells in the CNS determined. The levels of infectious TMEV, as measured by plaque assay at 3, 7, 14, and 28 days after infection were between 4 and 6 log10 PFU of virus per gram of CNS tissues at days 3 and 7 post-infection, and then decreased to undetectable levels by day 14 after infection in both strains of mice. The L-sel-/- mice had decreased numbers of CD8+ T lymphocytes (17.72% ± 2.4) infiltrating into the CNS at 7 days post-infection when compared to wild-type mice (31.02% ± 7.5). In addition, the L-sel-/- mice had significantly lower levels of TMEV-specific serum IgG resulting in lower virus neutralizing activity of the serum when compared to wild-type mice. However, the L-sel-/- mice had 2.5-fold increase in B lymphocytes in the CNS (8.29% ± 1.1) when compared to wild-type mice (3.2% ± 0.4). Taken together, these data indicate that L-selectin plays a role in recruitment of B and CD8+ T lymphocytes into the CNS following virus infection, which, however, did not affect the ability of the mice to clear TMEV infection.

KW - Central nervous system

KW - Demyelination

KW - L-selectin

KW - Lymphocytes

KW - Theiler's virus

UR - http://www.scopus.com/inward/record.url?scp=0035364047&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035364047&partnerID=8YFLogxK

U2 - 10.1016/S0165-5728(01)00296-X

DO - 10.1016/S0165-5728(01)00296-X

M3 - Article

C2 - 11438172

AN - SCOPUS:0035364047

VL - 116

SP - 178

EP - 187

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

SN - 0165-5728

IS - 2

ER -