The Water Channel Aquaporin-8 is Mainly Intracellular in Rat Hepatocytes, and its Plasma Membrane Insertion is Stimulated by Cyclic AMP

Fabiana García, Arlinet Kierbel, M. Cecilia Larocca, Sergio A. Gradilone, Patrick Splinter, Nicholas F La Russo, Raúl A. Marinelli

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Abstract

We previously found that water transport across hepatocyte plasma membranes occurs mainly via a non-channel mediated pathway. Recently, it has been reported that mRNA for the water channel, aquaporin-8 (AQP8), is present in hepatocytes. To further explore this issue, we studied protein expression, subcellular localization, and regulation of AQP8 in rat hepatocytes. By subcellular fractionation and immunoblot analysis, we detected an N-glycosylated band of ∼34 kDa corresponding to AQP8 in hepatocyte plasma and intracellular microsomal membranes. Confocal immunofluorescence microscopy for AQP8 in cultured hepatocytes showed a predominant intracellular vesicular localization. Dibutyryl cAMP (Bt2cAMP) stimulated the redistribution of AQP8 to plasma membranes. Bt2cAMP also significantly increased hepatocyte membrane water permeability, an effect that was prevented by the water channel blocker dimethyl sulfoxide. The microtubule blocker colchicine but not its inactive analog lumicolchicine inhibited the Bt2cAMP effect on both AQP8 redistribution to cell surface and hepatocyte membrane water permeability. Our data suggest that in rat hepatocytes AQP8 is localized largely in intracellular vesicles and can be redistributed to plasma membranes via a microtubule-depending, cAMP-stimulated mechanism. These studies also suggest that aquaporins contribute to water transport in cAMP-stimulated hepatocytes, a process that could be relevant to regulated hepatocyte bile secretion.

Original languageEnglish (US)
Pages (from-to)12147-12152
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number15
DOIs
StatePublished - Apr 13 2001

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Aquaporins
Cell membranes
Cyclic AMP
Rats
Hepatocytes
Cell Membrane
Water
Membranes
Microtubules
Permeability
aquaporin 8
Intracellular Membranes
Confocal microscopy
Colchicine
Fractionation
Dimethyl Sulfoxide
Fluorescence Microscopy
Confocal Microscopy
Bile
Plasmas

ASJC Scopus subject areas

  • Biochemistry

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The Water Channel Aquaporin-8 is Mainly Intracellular in Rat Hepatocytes, and its Plasma Membrane Insertion is Stimulated by Cyclic AMP. / García, Fabiana; Kierbel, Arlinet; Larocca, M. Cecilia; Gradilone, Sergio A.; Splinter, Patrick; La Russo, Nicholas F; Marinelli, Raúl A.

In: Journal of Biological Chemistry, Vol. 276, No. 15, 13.04.2001, p. 12147-12152.

Research output: Contribution to journalArticle

García, Fabiana ; Kierbel, Arlinet ; Larocca, M. Cecilia ; Gradilone, Sergio A. ; Splinter, Patrick ; La Russo, Nicholas F ; Marinelli, Raúl A. / The Water Channel Aquaporin-8 is Mainly Intracellular in Rat Hepatocytes, and its Plasma Membrane Insertion is Stimulated by Cyclic AMP. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 15. pp. 12147-12152.
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