The use of protein tyrosine phosphatase 1B and insulin receptor immunostains to differentiate nonalcoholic from alcoholic steatohepatitis in liver biopsy specimens

Schuyler O. Sanderson, Thomas Christopher Smyrk

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Nonalcoholic steatohepatitis (NASH) and alcoholic steatohepatitis (ASH) typically are indistinguishable histologically. The diagnosis relies on reporting of alcohol consumption. The metabolic syndrome involving insulin resistance is associated with nonalcoholic fatty liver disease (NAFLD). Protein tyrosine phosphatase 1B (PTP1B) negatively regulates the insulin receptor (IR). Increased PTP1B expression is seen in obesity and possibly is responsible for the insulin resistance seen in the metabolic syndrome. The study objective was to determine whether biopsy specimens with steatohepatitis could be classified accurately as alcoholic or nonalcoholic by immunohistochemical stains. We selected 241 cases of steatohepatitis, comprising 53 and 188 cases of alcoholic and NAFLD, respectively. Specimens were stained with PTP1B and IR (β subunit) and classified as NASH or ASH. The staining pattern predicted 60 cases of ASH and 181 cases of NASH. Results correlated with clinical diagnoses in 70% and 88% of ASH and NASH cases, respectively (odds ratio, 16.6; 95% confidence interval, 8.2-35.4).

Original languageEnglish (US)
Pages (from-to)503-509
Number of pages7
JournalAmerican Journal of Clinical Pathology
Volume123
Issue number4
DOIs
StatePublished - Apr 2005

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Non-Receptor Type 1 Protein Tyrosine Phosphatase
Alcoholic Fatty Liver
Insulin Receptor
Biopsy
Liver
Fatty Liver
Insulin Resistance
Alcohol Drinking
Non-alcoholic Fatty Liver Disease
Coloring Agents
Obesity
Odds Ratio
Confidence Intervals
Staining and Labeling

Keywords

  • Cirrhosis
  • Immunohistochemistry
  • Insulin resistance
  • NASH
  • Nonalcoholic Steatohepatitis
  • Obesity

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

The use of protein tyrosine phosphatase 1B and insulin receptor immunostains to differentiate nonalcoholic from alcoholic steatohepatitis in liver biopsy specimens. / Sanderson, Schuyler O.; Smyrk, Thomas Christopher.

In: American Journal of Clinical Pathology, Vol. 123, No. 4, 04.2005, p. 503-509.

Research output: Contribution to journalArticle

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