The use of microcomputed tomography to study microvasculature in small rodents

Michael D. Bentley, Maria C. Ortiz, Erik L. Ritman, J. Carlos Romero

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Appropriate nephron function is dependent on the intrarenal arrangement of blood vessels. The preferred and primary means to study the architecture of intrarenal circulation has been by filling it with opaque substances such as india ink, radio-opaque contrast material, or various polymers for study by light or scanning electron microscopy. With such methodologies, superficial vessels may obscure deep vessels and little quantitative information may be obtained. Serial-section microtomy has not been practical because of problems relating to alignment and registration of adjacent sections, lost sections, and preparation time and effort. Microcomputed tomography (micro-CT) overcomes such limitations and provides a means to study the three-dimensional architecture of filled vessels within an intact rodent kidney and to obtain more quantitative information. As an example of micro-CT's capabilities, we review the use of micro-CT to study the alterations in renal microvasculature caused by the development of liver cirrhosis after chronic bile duct ligation. In this example, micro-CT evidence shows a selective decrease in cortical vascular filling in the kidney, with a maintenance of medullary vascular filling. These changes may contribute to the salt and water retention that accompanies cirrhosis. These results indicate that micro-CT is a promising method to evaluate renal vascular architecture in the intact rodent kidney relative to physiological and pathological function.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume282
Issue number5 51-5
StatePublished - 2002

Fingerprint

X-Ray Microtomography
Microvessels
Rodentia
Blood Vessels
Kidney
Microtomy
Nephrons
Bile Ducts
Liver Cirrhosis
Electron Scanning Microscopy
Contrast Media
Ligation
Polymers
Fibrosis
Salts
Maintenance
Light
Water

Keywords

  • Imaging
  • Kidney
  • Microcirculation
  • Vasculature

ASJC Scopus subject areas

  • Physiology

Cite this

The use of microcomputed tomography to study microvasculature in small rodents. / Bentley, Michael D.; Ortiz, Maria C.; Ritman, Erik L.; Carlos Romero, J.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 282, No. 5 51-5, 2002.

Research output: Contribution to journalArticle

Bentley, Michael D. ; Ortiz, Maria C. ; Ritman, Erik L. ; Carlos Romero, J. / The use of microcomputed tomography to study microvasculature in small rodents. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2002 ; Vol. 282, No. 5 51-5.
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