The type 1 equilibrative nucleoside transporter regulates anxiety-like behavior in mice

J. Chen, L. Rinaldo, S. J. Lim, H. Young, R. O. Messing, Doo Sup Choi

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Activation of adenosine receptors in the brain reduces anxiety-like behavior in animals and humans. Because nucleoside transporters regulate adenosine levels, we used mice lacking the type 1 equilibrative nucleoside transporter (ENT1) to investigate whether ENT1 contributes to anxiety-like behavior. The ENT1 null mice spent more time in the center of an open field compared with wild-type littermates. In the elevated plus maze, ENT1 null mice entered more frequently into and spent more time exploring the open arms. The ENT1 null mice also spent more time exploring the light side of a light-dark box compared with wild-type mice. Microinjection of an ENT1-specific antagonist, nitrobenzylthioinosine (nitrobenzylmercaptopurine riboside), into the amygdala of C57BL/6J mice reduced anxiety-like behavior in the open field and elevated plus maze. These findings show that amygdala ENT1 modulates anxiety-like behavior. The ENT1 may be a drug target for the treatment of anxiety disorders.

Original languageEnglish (US)
Pages (from-to)776-783
Number of pages8
JournalGenes, Brain and Behavior
Volume6
Issue number8
DOIs
StatePublished - Nov 2007

Fingerprint

Equilibrative Nucleoside Transporter 1
Anxiety
Amygdala
Nucleoside Transport Proteins
Light
Animal Behavior
Purinergic P1 Receptors
Microinjections
Anxiety Disorders
Inbred C57BL Mouse
Adenosine
Brain
Pharmaceutical Preparations

Keywords

  • Amygdala
  • Behavioral genetics
  • Knockout
  • Nucleoside transporter
  • Psychiatric disorders

ASJC Scopus subject areas

  • Neuroscience(all)
  • Genetics

Cite this

The type 1 equilibrative nucleoside transporter regulates anxiety-like behavior in mice. / Chen, J.; Rinaldo, L.; Lim, S. J.; Young, H.; Messing, R. O.; Choi, Doo Sup.

In: Genes, Brain and Behavior, Vol. 6, No. 8, 11.2007, p. 776-783.

Research output: Contribution to journalArticle

Chen, J. ; Rinaldo, L. ; Lim, S. J. ; Young, H. ; Messing, R. O. ; Choi, Doo Sup. / The type 1 equilibrative nucleoside transporter regulates anxiety-like behavior in mice. In: Genes, Brain and Behavior. 2007 ; Vol. 6, No. 8. pp. 776-783.
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